N2912

Anti-Neurofilament 160/200 antibody, Mouse monoclonal

Name: Anti-Neurofilament 160/200 antibody, Mouse monoclonal
Brand: SIGMA

~2 mg/mL, clone RMdO20, purified from hybridoma cell culture

Synonym(s):

Anti-NEF3

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00164708

Product Name

Anti-Neurofilament 160/200 antibody, Mouse monoclonal, ~2 mg/mL, clone RMdO20, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

RMdO20, monoclonal

form

buffered aqueous solution

species reactivity

mouse, rat, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using extracts of rat brain S1 fraction.

isotype

IgG1

UniProt accession no.

P07197

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... NEFH(4744), NEFM(4741)
mouse ... Nefh(380684), Nefm(18040)
rat ... Nefh(24587), Nefm(24588)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Monoclonal anti-neurofilament 160/200 antibody can be used in immunocytochemistry (diluted 1: 100) and western blotting (diluted 1: 1,000) . It can also be used in immunohistochemistry.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neurofilaments are 10 nm in diameter and belong to intermediate filament protein family. Neurofilaments are expressed mainly in cells or tissues of neuronal origin and are crucial for proper radial growth of axons. Monoclonal anti-neurofilament 160/200 antibody can be used in ELISA and immunoblotting. It can also be used in microarray. Mouse anti-neurofilament 160/200 antibody reacts specifically with the non-phosphorylated form of NF-M and NF-H (NF-M/H, approx.160 and 200 kDa) in mouse, rat and human.

Immunogen

purified mid-size rat neurofilament (NF-M) subunit.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Dynamic changes in hippocampal diffusion and kurtosis metrics following experimental mTBI correlate with glial reactivity.

Kim Braeckman et al.

NeuroImage. Clinical, 21, 101669-101669 (2019-01-20)

Diffusion magnetic resonance imaging biomarkers can provide quantifiable information of the brain tissue after a mild traumatic brain injury (mTBI). However, the commonly applied diffusion tensor imaging (DTI) model is not very specific to changes in the underlying cellular structures.

Improved axonal regeneration of transected spinal cord mediated by multichannel collagen conduits functionalized with neurotrophin-3 gene.

L Yao et al.

Gene therapy, 20(12), 1149-1157 (2013-07-26)

Functionalized biomaterial scaffolds targeted at improving axonal regeneration by enhancing guided axonal growth provide a promising approach for the repair of spinal cord injury. Collagen neural conduits provide structural guidance for neural tissue regeneration, and in this study it is

Mrpl10 and Tbp Are Suitable Reference Genes for Peripheral Nerve Crush Injury.

Yaxian Wang et al.

International journal of molecular sciences, 18(2) (2017-01-31)

Peripheral nerve injury triggers the dysregulation of a large number of genes at multiple sites, including neurons, peripheral nerve stump, and the target organ. Housekeeping genes were frequently used as reference genes to normalize the expression values of target genes.

Monoclonal antibodies distinguish several differentially phosphorylated states of the two largest rat neurofilament subunits (NF-H and NF-M) and demonstrate their existence in the normal nervous system of adult rats.

V M Lee et al.

The Journal of neuroscience : the official journal of the Society for Neuroscience, 7(11), 3474-3488 (1987-11-01)

A new panel of greater than 300 monoclonal antibodies (mAbs) was prepared to the high, middle, and low Mr rat neurofilament (NF) subunits (NF-H, NF-M and NF-L, respectively). NF proteins were purified both from native, i.e., phosphorylated rat NFs and

MS-275, a class I histone deacetylase inhibitor, protects the p53-deficient mouse against ischemic injury.

Sean P Murphy et al.

Journal of neurochemistry, 129(3), 509-515 (2013-10-24)

The administration of pan histone deacetylase (HDAC) inhibitors reduces ischemic damage to the CNS, both in vitro and in animal models of stroke, via mechanisms which we are beginning to understand. The acetylation of p53 is regulated by Class I

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