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Choisissez des Panels configurables & des Kits préconfigurés - OU - des MAPmate™ de signalisation cellulaire
Concevez vos kits MILLIPLEX® MAP et obtenez leur prix.
Panels configurables & Kits préconfigurés
Notre large gamme est constituée de panels multiplex qui vous permettent de choisir, au sein d'un panel, les analytes qui répondent le mieux à vos besoins. Sur un autre onglet, vous pouvez choisir un format cytokine préconfiguré ou un kit Simplex.
Kits de signalisation cellulaire & MAPmate™
Choisissez des kits préconfigurés qui permettent d'explorer l'ensemble des voies ou des processus. Ou concevez vos propres kits en choisissant des Simplex MAPmate™ et en suivant les instructions fournies.
Les MAPmate™ suivants ne peuvent pas être utilisés ensemble : -des MAPmate™ qui nécessitent des tampons différents -des paires de MAPmate™ totaux et phospho-spécifiques, par ex. GSK3β total et GSK3β (Ser 9) -des MAPmate™ PanTyr et spécifiques d'un site, par ex. Récepteur Phospho-EGF et phospho-STAT1 (Tyr701) -Plus d'un phospho-MAPmate™ pour une seule cible (Akt, STAT3). -GAPDH et β-Tubuline ne peuvent pas être utilisés avec les kits ou les MAPmate™ contenant panTyr.
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Sélectionner une espèce, un type de panel, un kit ou un type d'échantillon
Pour commencer à concevoir votre kit MILLIPLEX® MAP, sélectionnez une espèce, un type de panel ou un kit d'intérêt.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Ajouter des réactifs supplémentaires (Un kit "Buffer and Detection Kit" est nécessaire pour une utilisation avec les MAPmate™)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Option de gain de place Nos clients qui commandent plusieurs kits peuvent choisir d'économiser de l'espace de stockage en éliminant l'emballage de chaque kit et de recevoir les composants de leur essai multiplex conditionnés sous poches en plastique pour un stockage plus compact.
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Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More
Our manufacturing facility in Cork, Ireland, is home to the patented process behind the Millex® filter's superior housing strength and filtration performance.
Cleanroom manufactured to pharmaceutical standards with fully automated equipment and 100% visual and physical inspection
Cleanroom classification: Millex® products are manufactured in ISO Class 8 Cleanroom as per ISO 14644-1
During the membrane cutting step, robotic inspection ensures that each membrane is cut precisely and accurately, for reliable performance. This quality control system analyzes and displays parameters for 16 membrane discs at a time.
Good manufacturing practice and ISO® 9001 Quality Standard: Millex® filters are manufactured in a facility whose Quality Management System is approved by an accredited registering body to the ISO® 9001 Quality Systems Standard
CE marking: Product is CE marked in accordance with EC directive 93/42/EEC
Quality Check (QC)
In-process integrity testing: Ensures that the product is made correctly and with a membrane fully sealed in the device
Vision testing for right placement of membrane
Air integrity testing
Pre-release testing: Provides customer with information which can impact application performance of a syringe filter
Flow rate / bubble point test
Burst testing
Salt particle challenge test
HPLC extractables test
IC extractables test
Comparing Millex® Filters to Other Syringe Filters
First of all, Millex® filters trap nearly 100% of particles from filtered samples, so that they never interfere with your sensitive downstream analysis.
Syringe filters from two different vendors (pore size 0.2 μm) were tested for latex particle retention following filtration of a suspension of 0.3 μm latex particles in water. (n = 3)
Also, higher sample recovery (lower hold-up volume) prevents loss of precious samples and ensures accurate results:
Lower hold-up volume prevents loss of precious samples. Graph shows comparison of recovery from 0.2 µm pore size PVDF membrane syringe filters. (Click image to enlarge.)
Quality Materials
With decades of expertise in membrane technology, we are known for our quality microfiltration and prefiltration materials. You can find detailed information about these materials and more in our Membrane Learning Center.
What other aspects of quality materials contribute to the high performance of Millex® filters?
Low-extractable housing
Very low levels of extractables from housing materials selected for Millex® filters is due to the broad, high chemical compatibility of both HDPE and PP materials.
Click images to enlarge.
Evaluation of the extractables profile of the housing materials of Millex® filters.
The experimental procedure for performing extractable analysis was as follows:
Syringe filter devices without the membrane were prepared and different solvents were filtered through it.
Following filtration, 1 mL fractions were collected and analyzed by reversed phase chromatography using a C18 column and a water/ acetonitrile gradient from 0-100% acetonitrile.
No peak was observed in the chromatogram indicating absence of any extractables.
The peak observed at about 2 min. is a solvent front / void volume peak whereas peak observed at 17 min. happens due to end of gradient switch to 0% acetonitrile from 100% acetonitrile.
Typically sample is not dissolved in exactly the same solvent as the mobile phase, and the solvent component from the injected sample elutes out first as it is not retained at all by the column. Since the solvent might have a slightly different absorbance at the detection wavelength (214nm in our example) a small peak may appear at the solvent front.
Similarly, when the gradient switches from 100% organic solvent to 100% aqueous sample, there is a sudden change in absorbance, which shows up as a peak at the end of the chromatogram. In normal chromatography the goal is to always stay away from the solvent front (because otherwise there is no retention and separation) and away from the end of gradient (because there is too much retention). The goal is to elute the analyte in the middle portion of the chromatogram.
HPLC-Certified Filters: Not Necessarily Clean
Note that all HPLC-certified filters are not equally clean. HPLC-certified filters from different vendors are all labeled as having “low levels of extractables.” Because methods of extractables testing differ between different vendors, a result that appears low under certain testing conditions can be higher under other HPLC conditions.
As can be clearly seen from the chromatograms below, all these HPLC-certified syringe filters show very different extractables profiles. The hydrophilic PTFE syringe filters show the least amount of extractables whereas the nylon syringe filters show very strong peaks between 4 – 6 min. On the other hand, the polypropylene membrane shows a broad peak between 4 – 7 min. This elution time range can be the critical range during which analytes of interest elute, and the presence of these extractables makes quantitation and identification much more difficult.
In the extractables analysis experiment shown, 70% acetonitrile in water was filtered through various syringe filters and the first mL of the filtrate was analyzed by reversed phase HPLC with UV detector set to 214 nm. HPLC was carried out using a C18 column and 0 – 100% acetonitrile gradient to ensure elution of all the impurities / extractables from the column. (Click image to enlarge.)