FAK100 Actin Cytoskeleton / Focal Adhesion Staining Kit

100 assays  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
Demander le prix
Disponibilité limitéeDisponibilité limitée
En stock 
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service


      Contacter le Service Clients


      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityKey Applications
      B, H, Po, Rb ICC
      Catalogue NumberFAK100
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionActin Cytoskeleton / Focal Adhesion Staining Kit
      OverviewThe Actin Cytoskeleton and Focal Adhesion Staining Kit consists of three components (TRITC-conjugated phalloidin, anti-Vinculin and DAPI) for the immunofluorescent staining of actin filaments in the cytoskeleton, focal contacts as well as the nucleus of the cells.

      Reagents and materials supplied in the Actin Cytoskeleton and Focal Adhesion Staining Kit are sufficient for 100 tests (including controls).


      The actin cytoskeleton is a highly dynamic network composed of actin polymers and a large variety of associated proteins. The functions of the actin cytoskeleton is to mediate a variety of essential biological functions in all eukaryotic cells, including intra- and extra-cellular movement and structural support. To perform these functions, the organization of the actin cytoskeleton must be tightly regulated both temporally and spatially. Many proteins associated with the actin cytoskeleton are thus likely targets of signaling pathways controlling actin assembly. Actin cytoskeleton assembly is regulated at multiple levels, including the organization of actin monomers (G-protein) into actin polymers and the superorganization of actin polymers into a filamentous network (F-actin - the major constituent of microfilaments) [1]. This superorganization of actin polymers into a filamentous network is mediated by actin side-binding or cross-linking proteins [2, 3, 4]. Actin cytoskeleton is a dynamic structure that rapidly changes shape and organization in reponse to stimuli and cell cycle progression. Therefore, a disruption of its normal regulation may lead to cell transformations, hence cancer. Transformed cells have been shown to contain less F-actin than untransformed cells and exhibit atypical coordination of F-actin levels to the cell cycle [5]. Orientational distribution of actin filaments within a cell is an important determinant of cellular shape and motility. Therefore,

      Focal adhesion and adherens junctions are membrane-associated complexes that serve as nucleation sites for actin filaments and as cross-linkers between the cell exterior, plasma membrane and actin cytoskeleton [6]. The function of focal adhesions are structural, linking the ECM on the outside to the actin cytoskeleton on the inside. They are also sites of signal transduction, initiating of signaling pathways in response to adhesion. Focal adhesions consist of integrin-type receptors that are attached to the extracellular matrix and are intracellularly associated with protein complexes containing vinculin (universal focal adhesion marker), talin, a-actinin, paxillin, tensin, zyxin and focal adhesion kinase (FAK) [7,8].

      CHEMICON's Actin Cytoskeleton and Focal Adhesion Staining Kit (Catalog number FAK100) is a very sensitive immunocytochemical tool that contains fluorescent-labeled phalloidin (TRITC-conjugated phalloidin) to map the local orientation of actin filaments within cell and a mouse monoclonal antibody to Vinculin that is very specific for the staining of focal contacts in cells.
      Materials Required but Not Delivered1. Fixative, e.g. 4% paraformaldehyde

      2. Permeabilizing reagent, e.g. 0.1% Triton X-100

      3. Blocking solution (1% BSA in 1xPBS)

      4. Fluorescent-labeled Secondary antibodies (For example CHEMICON Cat. No. AP124F)

      5. 1 x Wash Buffer (e.g. 1xPBS containing 0.05% Tween-20)

      6. Antifade mounting solution (For example: CHEMICON Cat. No. 5013)

      7. Glass slides and coverslips
      Product Information
      • Vinculin Monoclonal antibody, purified clone 7F9 (Part No. 90227). One vial containing 100 μL at 1 mg/mL.
      • TRITC-conjugated Phalloidin (Part No. 90228). One vial containing 15 μg.
      • DAPI (Part No. 90229). One vial containing 100 μL.
      Quality LevelMQ100
      ApplicationThe Actin Cytoskeleton & Focal Adhesion Staining Kit consists of TRITC-conjugated phalloidin, anti-Vinculin & DAPI for the immunofluorescent staining of actin filaments in the cytoskeleton as well as the nucleus of the cells.
      Key Applications
      • Immunocytochemistry
      Biological Information
      Species Reactivity
      • Bovine
      • Human
      • Pig
      • Rabbit
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsWhen stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
      Packaging Information
      Material Size100 assays
      Transport Information
      Supplemental Information


      Actin Cytoskeleton / Focal Adhesion Staining Kit FDS


      Fiche de données de sécurité des matériaux (FDS) 

      Références bibliographiques

      Aperçu de la référence bibliographiqueNº PubMed
      TIMP-1 Induces an EMT-Like Phenotypic Conversion in MDCK Cells Independent of Its MMP-Inhibitory Domain.
      Young Suk Jung,Xu-Wen Liu,Rosemarie Chirco,Richard B Warner,Rafael Fridman,Hyeong-Reh Choi Kim
      PloS one  7  2012

      Afficher le résumé
      22701711 22701711
      Effect of substrate stiffness on pulmonary fibroblast activation by TGF-β.
      H N Chia,M Vigen,A M Kasko
      Acta biomaterialia  8  2012

      Afficher le résumé
      22446029 22446029
      How to assess cytotoxicity of (iron oxide-based) nanoparticles: a technical note using cationic magnetoliposomes.
      Stefaan J H Soenen,Marcel De Cuyper
      Contrast media & molecular imaging  6  2011

      Afficher le résumé
      21698773 21698773
      Mitochondria-targeted peptide MTP-131 alleviates mitochondrial dysfunction and oxidative damage in human trabecular meshwork cells.
      Min Chen,Bingqian Liu,Qianying Gao,Yehong Zhuo,Jian Ge
      Investigative ophthalmology & visual science  52  2011

      Afficher le résumé
      21697135 21697135
      A novel shell-structure cell microcarrier (SSCM) for cell transplantation and bone regeneration medicine.
      Kai Su,Yihong Gong,Chunming Wang,Dong-An Wang
      Pharmaceutical research  28  2011

      Afficher le résumé
      21088984 21088984
      Balance of life and death in alveolar epithelial type II cells: proliferation, apoptosis, and the effects of cyclic stretch on wound healing.
      Lynn M Crosby,Charlean Luellen,Zhihong Zhang,Larry L Tague,Scott E Sinclair,Christopher M Waters
      American journal of physiology. Lung cellular and molecular physiology  301  2011

      Afficher le résumé
      21724858 21724858
      Manipulating location, polarity, and outgrowth length of neuron-like pheochromocytoma (PC-12) cells on patterned organic electrode arrays.
      Yu-Sheng Hsiao,Chung-Chih Lin,Hsin-Jui Hsieh,Shih-Min Tsai,Chiung-Wen Kuo,Chih-Wei Chu,Peilin Chen
      Lab on a chip  11  2011

      Afficher le résumé
      21922117 21922117
      Cell culture and motility study on a polymer surface with a nanometer-scaled stripe structure.
      Sakamoto Y, Sato K, Abo M, Tsukahara T, Kitamori T, Abe K, Yoshimura E
      Biosci Biotechnol Biochem  74  569-72. Epub 2010 Mar 7.  2010

      Afficher le résumé
      20208350 20208350
      High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling.
      Soenen SJ, Nuytten N, De Meyer SF, De Smedt SC, De Cuyper M
      Small  6  832-42.  2010

      Afficher le résumé
      20213651 20213651
      In vitro cellular response and in vivo primary osteointegration of electrochemically modified titanium.
      F Ravanetti, P Borghetti, E De Angelis, R Chiesa, FM Martini, C Gabbi, A Cacchioli
      Acta biomaterialia  6  1014-24  2010

      Afficher le résumé
      19800423 19800423

      Fiche technique

      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

      Manuels d'utilisation

      Actin Cytoskeleton and Focal Adhesion Staining Kit

      Newsletters / Publications

      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011

      Produits & Applications associés

      Familles de produits


      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunohistochemistry (IHC) > Histological Stains and Staining Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunocytochemistry (ICC) > Immunocytochemistry Reagents
      Life Science Research > Antibodies and Assays > Immunoassays > Immunohistochemistry (IHC) > Histological Stains and Staining Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Immunocytochemistry (ICC) > Immunocytochemistry Reagents