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07-414 Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24)

200 µL  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbSerumPolyclonal Antibody
Catalogue Number07-414
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-dimethyl-Arginine Antibody, asymmetric (ASYM24)
Background InformationArginine methylation is a post-translational modification that results in the formation of asymmetrical and symmetrical dimethylated arginines (a- and sDMA).
Product Information
  • Jurkat cell lysate was probed with Anti-dimethyl-Arginine, asymmetric. Several proteins containing asymmetrically dimethylated arginines were detected, including p110, p75 and Sam68.

    Included Positive Antigen Control:
    Catalog # 12-303, Jurkat lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
PresentationRabbit antiserum diluted in storage buffer (PBS, 0.035% sodium azide and 30% glycerol). Store at -20°C.
Quality LevelMQ100
ApplicationDetect dimethyl-Arginine with Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) (Rabbit Polyclonal Antibody), that has been shown to work in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide containing asymmetric dimethyl-arginine-glycine repeats (KGR*GR*GR*GR*GPPPPPR*GR*GR*GR*G in which R* denotes asymmetric dimethylation of arginine).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityRecognizes proteins that contain arginines that are asymmetrically dimethylated including p300, p110, p75 and Sam68. Specificity for asymmetrically dimethylated arginine was determined by ELISA with an unmethylated ‘backbone’ peptide or an identical peptide containing symmetrically dimethylated arginines.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman and mouse. Reactivity with other species has not been confirmed.
Antibody TypePolyclonal Antibody
  • Methylation
Purification MethodUnpurified
Molecular WeightVaries. Antibody detects proteins containing asymmetrical dimethylated arginines.
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot HeLa nuclear extract and p110, p75, and Sam68 in RIPA lysates from Jukat cells.

Western Blot Analysis:
A 1:500-1:1000 dilution of this lot detected p110, p75, and Sam68 in RIPA lysates from Jurkat cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Global Trade Item Number
Référence GTIN
07-414 04053252373558


Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) FDS


Fiche de données de sécurité des matériaux (FDS) 

Anti-dimethyl-Arginine Antibody, asymmetric (ASYM24) Certificats d'analyse

TitreNuméro de lot
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2387832 2387832
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2453240 2453240
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2459628 2459628
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 0704057819 0704057819
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2168338 2168338
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2250514 2250514
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 2327463 2327463
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 24346 24346
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 30149 30149
Anti-dimethyl-Arginine, asymmetric (ASYM24) - 3027216 3027216

Références bibliographiques

Aperçu de la référence bibliographiqueApplicationEspèceNº PubMed
LXR-Mediated ABCA1 Expression and Function Are Modulated by High Glucose and PRMT2.
Hussein, MA; Shrestha, E; Ouimet, M; Barrett, TJ; Leone, S; Moore, KJ; Hérault, Y; Fisher, EA; Garabedian, MJ
PloS one  10  e0135218  2015

Afficher le résumé
26288135 26288135
JMJD6 regulates ERα methylation on arginine.
Poulard, C; Rambaud, J; Hussein, N; Corbo, L; Le Romancer, M
PloS one  9  e87982  2014

Afficher le résumé
24498420 24498420
5-Hydroxymethylcytosine Plays a Critical Role in Glioblastomagenesis by Recruiting the CHTOP-Methylosome Complex.
Takai, H; Masuda, K; Sato, T; Sakaguchi, Y; Suzuki, T; Suzuki, T; Koyama-Nasu, R; Nasu-Nishimura, Y; Katou, Y; Ogawa, H; Morishita, Y; Kozuka-Hata, H; Oyama, M; Todo, T; Ino, Y; Mukasa, A; Saito, N; Toyoshima, C; Shirahige, K; Akiyama, T
Cell reports  9  48-60  2014

Afficher le résumé
25284789 25284789
Amyotrophic lateral sclerosis-linked FUS/TLS alters stress granule assembly and dynamics.
Baron, DM; Kaushansky, LJ; Ward, CL; Sama, RR; Chian, RJ; Boggio, KJ; Quaresma, AJ; Nickerson, JA; Bosco, DA
Molecular neurodegeneration  8  30  2013

Afficher le résumé
Immunocytochemistry24090136 24090136
Myofibrillar Ca(2+) sensitivity is uncoupled from troponin I phosphorylation in hypertrophic obstructive cardiomyopathy due to abnormal troponin T.
Bayliss, CR; Jacques, AM; Leung, MC; Ward, DG; Redwood, CS; Gallon, CE; Copeland, O; McKenna, WJ; Dos Remedios, C; Marston, SB; Messer, AE
Cardiovascular research  97  500-8  2013

Afficher le résumé
23097574 23097574
Protein arginine methyltransferase 1 and 8 interact with FUS to modify its sub-cellular distribution and toxicity in vitro and in vivo.
Scaramuzzino, C; Monaghan, J; Milioto, C; Lanson, NA; Maltare, A; Aggarwal, T; Casci, I; Fackelmayer, FO; Pennuto, M; Pandey, UB
PloS one  8  e61576  2013

Afficher le résumé
Western Blotting23620769 23620769
FUS/TLS assembles into stress granules and is a prosurvival factor during hyperosmolar stress.
Sama, RR; Ward, CL; Kaushansky, LJ; Lemay, N; Ishigaki, S; Urano, F; Bosco, DA
Journal of cellular physiology  228  2222-31  2013

Afficher le résumé
Immunofluorescence23625794 23625794
PRMT1 interacts with AML1-ETO to promote its transcriptional activation and progenitor cell proliferative potential.
Shia, WJ; Okumura, AJ; Yan, M; Sarkeshik, A; Lo, MC; Matsuura, S; Komeno, Y; Zhao, X; Nimer, SD; Yates, JR; Zhang, DE
Blood  119  4953-62  2011

Afficher le résumé
22498736 22498736
The effect of PRMT1-mediated arginine methylation on the subcellular localization, stress granules, and detergent-insoluble aggregates of FUS/TLS.
Yamaguchi, A; Kitajo, K
PloS one  7  e49267  2011

Afficher le résumé
Immunofluorescence23152885 23152885
Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.
Fanis, P; Gillemans, N; Aghajanirefah, A; Pourfarzad, F; Demmers, J; Esteghamat, F; Vadlamudi, RK; Grosveld, F; Philipsen, S; van Dijk, TB
Molecular & cellular proteomics : MCP  11  1263-73  2011

Afficher le résumé
Western BlottingHuman22872859 22872859


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