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07-520 Anti-eNOS/NOS III Antibody, CT

200 µg  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, B, M, Ca, Gp, PoWBRbPurifiedPolyclonal Antibody
Catalogue Number07-520
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-eNOS/NOS III Antibody, CT
OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
Alternate Names
  • Constitutive NOS
  • Endothelial NOS
  • Endothelial NOS
  • Endothelial NOS
  • endothelial nitric oxide synthase
  • nitric oxide synthase 3 (endothelial cell)
Background InformationEndothelial NOS (eNOS), also known as Nitric oxide synthase 3 (NOS3) or constitutive NOS (cNOS), is a nitric oxide synthase. It generates NO in blood vessels and is involved with regulating vascular function. A constitutive Ca2+ dependent NOS provides a basal release of NO. eNOS is associated with plasma membranes surrounding cells and the membranes of Golgi bodies within cells.
Product Information
HS Code3002 15 90
  • Platelets, placenta, liver and kidney.
PresentationPurified rabbit polyclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide before the addition of glycerol to 30%. Liquid at -20°C.
Quality LevelMQ100
ApplicationAnti-eNOS/NOS III Antibody, CT detects level of eNOS/NOS III & has been published & validated for use in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, pig, rat, and dog.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityRecognizes eNOS/NOS III, Mr ~135 kDa.
Species Reactivity
  • Human
  • Rat
  • Bovine
  • Mouse
  • Canine
  • Guinea Pig
  • Pig
Species Reactivity NoteHuman and bovine.
Predicted to cross-react with mouse, guinea pig, pig, rat and dog based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOS3
  • cNOS
  • eNOS
  • EC-NOS
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1203 amino acids; 133289 Da
SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain. ... hide » see more »
Molecular Weight135 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot in RIPA lysates from HUVEC cells.

Western Blot Analysis:
0.5-2 µg/mL of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size200 µg
Transport Information
Supplemental Information
Global Trade Item Number
Référence GTIN
07-520 04053252372728


Anti-eNOS/NOS III Antibody, CT FDS


Fiche de données de sécurité des matériaux (FDS) 

Anti-eNOS/NOS III Antibody, CT Certificats d'analyse

TitreNuméro de lot
Anti-eNOS/NOS III, CT 2476971
Anti-eNOS/NOS III, CT (rabbit polyclonal IgG) - DAM1424997 DAM1424997
Anti-eNOS/NOS III, CT - 2203125 2203125
Anti-eNOS/NOS III, CT - 2293831 2293831
Anti-eNOS/NOS III, CT - 24650 24650
Anti-eNOS/NOS III, CT - 3013418 3013418
Anti-eNOS/NOS III, CT - 3277166 3277166
Anti-eNOS/NOS III, CT - 3365612 3365612
Anti-eNOS/NOS III, CT - 3930307 3930307
Anti-eNOS/NOS III, CT - 4107248 4107248

Références bibliographiques

Aperçu de la référence bibliographiqueEspèceNº PubMed
TJ0711, a novel vasodilatory β-blocker, protects SHR rats against hypertension induced renal injury.
Yang, Juan, et al.
Am J Transl Res, 5: 279-90 (2013)  2013

Afficher le résumé
23634239 23634239
Apigenin inhibits the TNFα-induced expression of eNOS and MMP-9 via modulating Akt signalling through oestrogen receptor engagement.
Daniela Palmieri,Patrizia Perego,Domenico Palombo
Molecular and cellular biochemistry  371  2011

Afficher le résumé
22899172 22899172
Fetal pulmonary vascular remodeling in a rat model induced by hypoxia and indomethacin.
Xue-Feng Xu,Wei-Zhong Gu,Xi-Ling Wu,Ru-Yi Li,Li-Zhong Du
The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians  24  2010

Afficher le résumé
20459333 20459333
Functional protein network activation mapping reveals new potential molecular drug targets for poor prognosis pediatric BCP-ALL.
Accordi, Benedetta, et al.
PLoS ONE, 5: e13552 (2010)  2009

Afficher le résumé
21042412 21042412
BDNF/TrkB content and interaction with gastrin-releasing peptide receptor blockade in colorectal cancer.
Caroline Brunetto de Farias,Denis Broock Rosemberg,Tiago Elias Heinen,Patricia Koehler-Santos,Ana Lucia Abujamra,Flávio Kapczinski,Algemir Lunardi Brunetto,Patricia Ashton-Prolla,Luise Meurer,Maurício Reis Bogo,Daniel C Damin,Gilberto Schwartsmann,Rafael Roesler
Oncology  79  2009

Afficher le résumé
21474968 21474968
The senescence-accelerated mouse (SAM-P8) as a model for the study of vascular functional alterations during aging.
Lloréns, Silvia, et al.
Biogerontology, 8: 663-72 (2007)  2007

Mouse17786580 17786580


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