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04-1479 Anti-methyl-PP2A Antibody, C subunit, clone 2A10

150 µg  
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Replacement Information
Replacement Information04-1479 is a recommended replacement for 05-546.

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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Yeast (S. cerevisiae), R, Po, Rb, Ch, DrWBMPurifiedMonoclonal Antibody
Catalogue Number04-1479
DescriptionAnti-methyl-PP2A Antibody, C subunit, clone 2A10
Background InformationType 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal down-modulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.
Product Information
  • Lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.
PresentationPurified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl and 0.05% sodium azide.
Quality LevelMQ100
ApplicationThis Anti-methyl-PP2A Antibody, C subunit, clone 2A10 is validated for use in WB for the detection of methyl-PP2A.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated, synthetic peptide (C-βA-RRTPDYFL-Ome) corresponding to amino acids 302-309 of human PP2A catalytic subunit. Clone 2A10.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
SpecificityRecognizes the methylated form of PP2A, catalytic subunit.
Species Reactivity
  • Human
  • Mouse
  • Yeast (S. cerevisiae)
  • Rat
  • Pig
  • Rabbit
  • Chicken
  • Drosophila
Species Reactivity NoteHuman, mouse and yeast. Predicted to cross react with rat, pig, rabbit, chicken and Drosophila.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
  • Methylation
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~36 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by western blot on lysates from a yeast wild type strain (strain lacking the methyltransferase, delta PPM1) and a strain lacking the methylestrase, delta PPE1.

Western Blot Analysis: 1-5 µg/mL of this antibody detected the methylated form of PP2A catalytic subunit in yeast lysates lacking the methylestrase (increased methylation). Treatment of the blot with 0.2 M NaOH will demethylate proteins, thus eliminating detection of PP2A.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Packaging Information
Material Size150 µg
Transport Information
Supplemental Information
Global Trade Item Number
Référence GTIN
04-1479 04053252477546


Anti-methyl-PP2A Antibody, C subunit, clone 2A10 FDS


Fiche de données de sécurité des matériaux (FDS) 

Anti-methyl-PP2A Antibody, C subunit, clone 2A10 Certificats d'analyse

TitreNuméro de lot
Anti-methyl-PP2A, C subunit, clone 2A10 - 2116004 2116004
Anti-methyl-PP2A, C subunit, clone 2A10 - 2146011 2146011
Anti-methyl-PP2A, C subunit, clone 2A10 - 2377651 2377651
Anti-methyl-PP2A, C subunit, -2680521 2680521
Anti-methyl-PP2A, C subunit, -2697391 2697391
Anti-methyl-PP2A, C subunit, -2757106 2757106
Anti-methyl-PP2A, C subunit, -2779130 2779130
Anti-methyl-PP2A, C subunit, Clone 2A10 - 4039819 4039819
Anti-methyl-PP2A, C subunit, Clone 2A10 - 4103083 4103083
Anti-methyl-PP2A, C subunit, clone 2A10 - 1983712 1983712

Références bibliographiques

Aperçu de la référence bibliographiqueApplicationNº PubMed
Altered protein phosphatase 2A methylation and Tau phosphorylation in the young and aged brain of methylenetetrahydrofolate reductase (MTHFR) deficient mice.
Sontag, JM; Wasek, B; Taleski, G; Smith, J; Arning, E; Sontag, E; Bottiglieri, T
Frontiers in aging neuroscience  6  214  2014

Afficher le résumé
Western Blotting25202269 25202269
Cytoplasmic SET induces tau hyperphosphorylation through a decrease of methylated phosphatase 2A.
Chasseigneaux, S; Clamagirand, C; Huguet, L; Gorisse-Hussonnois, L; Rose, C; Allinquant, B
BMC neuroscience  15  82  2014

Afficher le résumé
Western Blotting24981783 24981783
NNMT promotes epigenetic remodeling in cancer by creating a metabolic methylation sink.
Ulanovskaya, OA; Zuhl, AM; Cravatt, BF
Nature chemical biology  9  300-6  2013

Afficher le résumé
Western Blotting23455543 23455543
Protein phosphatase 2A carboxymethylation and regulatory B subunits differentially regulate mast cell degranulation.
Kranias, Gregory, et al.
Cell. Signal., 22: 1882-90 (2010)  2009

Afficher le résumé
20688157 20688157