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04-811 Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal

100 µL  
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Replacement Information
Replacement InformationCat. # 05-811. 05-811 will only be availabe on a custom basis.

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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
BWBRbCulture SupernatantMonoclonal Antibody
Catalogue Number04-811
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal
OverviewNote: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS
Alternate Names
  • endothelial nitric oxidase synthase
  • endothelial nitric oxide synthase
  • nitric oxide synthase 3 (endothelial cell)
Product Information
FormatCulture Supernatant
  • Fetal bovine heart endothelial cell lysate
PresentationCultured supernatant in 0.05% sodium azide
Quality LevelMQ100
ApplicationThis Anti-phospho-eNOS/NOS III (Thr495) Antibody is validated for use in WB for the detection of phospho-eNOS/NOS III (Thr495).
Key Applications
  • Western Blotting
Application NotesWestern Blot Analysis:
A 1:2,000-1:10,000 dilution of this lot detected phosphorylated eNOS/NOS III in fetal bovine heart endothelial cell lysate.
Biological Information
ImmunogenPeptide containing the sequence KK[pT]FK in which pT corresponds to phosphothreonine at residue 495 of human eNOS/NOS III
SpecificityRecognizes phosphorylated eNOS/NOS III.
Species Reactivity
  • Bovine
Species Reactivity NotePredicted cross-reactivity with human, rabbit, mouse and rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOS3
  • cNOS
  • eNOS
  • EC-NOS
  • Phosphorylation
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
SIZE: 1203 amino acids; 133289 Da
SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
Molecular Weight135 kDa
Physicochemical Information
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by immunoblot on fetal bovine heart endothelial cell lysate
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Global Trade Item Number
Référence GTIN
04-811 04053252368769


Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal FDS


Fiche de données de sécurité des matériaux (FDS) 

Anti-phospho-eNOS/NOS III (Thr495) Antibody, rabbit monoclonal Certificats d'analyse

TitreNuméro de lot
Anti-phospho-eNOS/NOS III (Thr495) 2840424
Anti-phospho-eNOS/NOS III (Thr495) 3172126
Anti-phospho-eNOS/NOS III (Thr495) - 2137052 2137052
Anti-phospho-eNOS/NOS III (Thr495) - 2492799 2492799
Anti-phospho-eNOS/NOS III (Thr495) - 3003697 3003697
Anti-phospho-eNOS/NOS III (Thr495) - 3999543 3999543
Anti-phospho-eNOS/NOS III (Thr495) - DAM1495797 DAM1495797
Anti-phospho-eNOS/NOS III (Thr495) - JBC1482108 JBC1482108
Anti-phospho-eNOS/NOS III (Thr495) - NG1873186 NG1873186
Anti-phospho-eNOS/NOS III (Thr495) -2743612 2743612

Références bibliographiques

Aperçu de la référence bibliographiqueNº PubMed
Novel protective role of endogenous cardiac myocyte P2X4 receptors in heart failure.
Yang, T; Shen, JB; Yang, R; Redden, J; Dodge-Kafka, K; Grady, J; Jacobson, KA; Liang, BT
Circulation. Heart failure  7  510-8  2014

Afficher le résumé
24622244 24622244


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