17-294 Rho Activation Assay Kit

1 kit  Kit capacity: 30 assays
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      Replacement Information

      Tableau de caractéristiques principal

      Key ApplicationsDetection Methods
      ABA Fluorescent, Chemiluminescence, Colorimetric
      Catalogue Number17-294
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionRho Activation Assay Kit
      OverviewA GST-tagged fusion protein, corresponding to residues 7-89 of mouse Rhotekin Rho Binding Domain, expressed in E. coli. Provided bound to glutathione-agarose. Purity >90%.
      Product Information
      • 100X GTPγS, 10mM (Cat.# 20-176)
      • 100X GDP, 100mM (Cat.# 20-177)
      • Mg2+ Lysis/Wash Buffer, 5X (Cat.# 20-168)
      • Anti-Rho (-A, -B, -C), clone 55 (Cat.# 05-778)
      • Rho Assay Reagent (Rhotekin RBD, agarose) (Cat.# 14-383)
      Detection methodFluorescent Chemiluminescence Colorimetric
      Quality LevelMQ100
      ApplicationPP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.
      Key Applications
      • Affinity Binding Assay
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
      Gene Symbol
      • RHOA
      • RHO12
      • Rho12
      • RhoA
      • ARHA
      • ARH12
      • RHOH12
      • H12
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62745 # Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development.
      SIZE: 196 amino acids; 22123 Da
      SUBUNIT: Binds ROCK1 and ROCK2. Also binds PKN1/PRK1. Interacts with ARGGEF3, RTKN and AKAP13.
      SUBCELLULAR LOCATION: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Note=Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus.
      PTM: Prenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome.
      SIMILARITY: SwissProt: P62745 ## Belongs to the small GTPase superfamily. Rho family.
      MISCELLANEOUS: RHOB is one of the targets of farnesyltransferase inhibitors which are currently under investigation as cancer therapeutics. These elevate the levels of geranylgeranylated RHOB and cause mislocalization, leading to apoptosis and antineoplastic effects.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by precipitating GTP- Rho from 3T3/A31 cell lysates, subsequent detection by immunoblot analysis using anti-Rho (05-778).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 30 assays
      Transport Information
      Supplemental Information


      Rho Activation Assay Kit FDS


      Fiche de données de sécurité des matériaux (FDS) 

      Rho Activation Assay Kit Certificats d'analyse

      TitreNuméro de lot
      Rho Activation Assay Kit2967029
      Rho Activation Assay Kit2470979
      Rho Activation Assay Kit2806024
      Rho Activation Assay Kit3059658
      Rho Activation Assay Kit3115430
      Rho Activation Assay Kit - 23904972390497
      Rho Activation Assay Kit - 06070345780607034578
      Rho Activation Assay Kit - 06110445360611044536
      Rho Activation Assay Kit - 06110464450611046445
      Rho Activation Assay Kit - 07010493440701049344

      Références bibliographiques

      Aperçu de la référence bibliographiqueApplicationNº PubMed
      Prostaglandin E2 Inhibits Human Lung Fibroblast Chemotaxis through Disparate Actions on Different E-Prostanoid Receptors.
      Li YJ, Wang XQ, Sato T, Kanaji N, Nakanishi M, Kim M, Michalski J, Nelson AJ, Sun JH, Farid M, Basma H, Patil A, Toews ML, Liu X, Rennard SI
      Am J Respir Cell Mol Biol  2010

      Afficher le résumé
      20203295 20203295
      Cyclin-dependent kinase 5 regulates endothelial cell migration and angiogenesis.
      Johanna Liebl,Sabine B Weitensteiner,György Vereb,Lili Takács,Robert Fürst,Angelika M Vollmar,Stefan Zahler
      The Journal of biological chemistry  285  2010

      Afficher le résumé Article en texte intégral
      20826806 20826806
      Attenuation of angiotensin II-induced hypertension and cardiac hypertrophy in transgenic mice overexpressing a type 1 receptor mutant.
      Saad Ahmad,Francesca Cesana,Edward Lamperti,Haralambos Gavras,Jun Yu
      American journal of hypertension  22  2009

      Afficher le résumé Article en texte intégral
      19779471 19779471
      Pulmonary microvascular endothelial cells form a tighter monolayer when grown in chronic hypoxia.
      Victor Solodushko, James C Parker, Brian Fouty
      American journal of respiratory cell and molecular biology  38  491-7  2008

      Afficher le résumé Article en texte intégral
      18048805 18048805
      Sphingosine 1-phosphate potentiates human lung fibroblast chemotaxis through the S1P2 receptor.
      Mitsu Hashimoto, Xingqi Wang, Lijun Mao, Tetsu Kobayashi, Shin Kawasaki, Naoyoshi Mori, Myron L Toews, Hui Jung Kim, D Roselyn Cerutis, Xiangde Liu, Stephen I Rennard
      American journal of respiratory cell and molecular biology  39  356-63  2008

      Afficher le résumé Article en texte intégral
      18367729 18367729
      Atorvastatin decreases lipoprotein lipase and endothelial lipase expression in human THP-1 macrophages.
      Guosong Qiu, John S Hill
      Journal of lipid research  48  2112-22  2007

      Afficher le résumé
      17644777 17644777
      Rho signalling at a glance.
      Schwartz, Martin
      J. Cell. Sci., 117: 5457-8 (2004)  2004

      15509861 15509861
      Protein kinase A-mediated phosphorylation of the Galpha13 switch I region alters the Galphabetagamma13-G protein-coupled receptor complex and inhibits Rho activation
      Manganello, J. M., et al
      J Biol Chem, 278:124-30 (2003)  2003

      Activation Assay12399457 12399457
      PTEN regulates RANKL- and osteopontin-stimulated signal transduction during osteoclast differentiation and cell motility
      Sugatani, T., et al
      J Biol Chem, 278:5001-8 (2003)  2003

      Activation Assay12460992 12460992
      Protein kinase involved in lung injury susceptibility: Evidence from enzyme isoform genetic knockout and in vivo inhibitor treatment
      Wainwright, M. S., et al
      Proc Natl Acad Sci U S A, 100:6233-8 (2003)  2003

      Activation Assay12730364 12730364


      Hallmarks of Aging

      Produits & Applications associés

      Kit Component

      Référence Description  
      05-778 Anti-Rho (-A Antibody, -B, -C), clone 55 Prix & Disponibilité
      14-383 Rho Assay Reagent (Rhotekin RBD, agarose), 650 µg Prix & Disponibilité
      20-168 Mg2+ Lysis/Wash Buffer, 5X Prix & Disponibilité
      20-176 100X GTPγS, 10mM Prix & Disponibilité
      20-177 100X GDP, 100mM Prix & Disponibilité


      Life Science Research > Protein Detection and Quantification > Activity Assays > Activation Assays