M8403
McCoy’s 5A Medium
With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
Synonyme(s) :
5A basal medium
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McCoy′s 5A Medium, Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Segment
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
L-glutamine: no
NaHCO3: 2.2 g/L
glucose: 3.0 g/L
phenol red: 0.011 g/L
shipped in
ambient
storage temp.
2-8°C
General description
McCoy′s 5A Medium is suitable for the primary culture of cells from a wide range of tissue. It is recommended for the production of viruses in primary cell cultures.
Application
McCoy′s 5A Medium has been used to maintain:
- G-361 melanoma cells;
- 87-MG cells
- human colon carcinoma cell line HCT116
Biochem/physiol Actions
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
Preparation Note
Supplement with 0.22 g/L L-glutamine.
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Classe de stockage
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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