- Improvement of the enzyme performance of trypsin via adsorption in mesoporous silica SBA-15: hydrolysis of BAPNA.
Improvement of the enzyme performance of trypsin via adsorption in mesoporous silica SBA-15: hydrolysis of BAPNA.
Molecules (Basel, Switzerland) (2013-01-18)
Shanshan Li, Zhuofu Wu, Ming Lu, Zhi Wang, Zhengqiang Li
PMID23325102
ABSTRACT
The enzymatic performance of trypsin in hydrolysis of N-α-benzoyl-DL-arginine-4-nitroanilide (BAPNA) was improved by adsorption on Santa Barbara Amorphous (SBA)-15 mesoporous silica. The optimal immobilization conditions were screened and the properties of immobilized enzyme have also been studied. Under the optimal conditions, the immobilized trypsin displays maximum specific activity (49.8 μmol/min/g). The results also indicate that the immobilized trypsin exhibits better storage stability.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Trypsin from bovine pancreas, TPCK Treated, essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein
Sigma-Aldrich
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