05-253
Anti-ZAP-70 Antibody, clone 2F3.2
clone 2F3.2, Upstate®, from mouse
Synonym(s):
Anti-ADMIO2, Anti-SRK, Anti-STCD, Anti-STD, Anti-TZK, Anti-ZAP-70
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-ZAP-70 Antibody, clone 2F3.2, clone 2F3.2, Upstate®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
2F3.2, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ZAP70(7535)
Related Categories
Physical form
Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Format: Purified
Protein G Purified
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysis Note
Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
routinely evaluated on RIPA lysates prepared from Jurkat cells or Hut-78 cells
Application
For testing in IHC, please see the following link (PDF): http://www.propathlab.com/pdf/focus_dec_2003.pdf
Research Category
Signaling
Signaling
Research Sub Category
Immunological Signaling
Immunological Signaling
This Anti-ZAP-70 Antibody, clone 2F3.2 is validated for use in FC, IH, IP, WB for the detection of ZAP-70.
Biochem/physiol Actions
Recognizes human ZAP-70, does not recognize murine ZAP-70.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
70 kDa
Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.
Zeta-chain-associated protein kinase 70. A member of the protein tyrosine kinase family, ZAP-70 is normally expressed in T cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 is expressed in T cells and tumors of T-cell lineage. A high level of ZAP-70 expression appears restricted to T-cell proliferative diseases and a subgroup of chronic lymphocytic leukemia (CLL). The ZAP-70 gene is in chromosome 2q12.
Immunogen
GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
C Silvin et al.
The Journal of biological chemistry, 276(24), 21450-21457 (2001-04-03)
Wiskott-Aldrich syndrome protein (WASP) plays a key role in cytoskeletal rearrangement and transcriptional activation in T-cells. Recent evidence links WASP and related proteins to actin polymerization by the Arp2/3 complex. To study whether the role of WASP in actin polymerization
Eva Calpe et al.
PloS one, 8(12), e81221-e81221 (2013-12-07)
ZAP-70 in chronic lymphocytic leukemia (CLL) is associated with enhanced response to microenvironmental stimuli. We analyzed the functional consequences of ZAP-70 ectopic expression in malignant B-cells in a xenograft mouse model of disseminated B-cell leukemia. Mice injected with B-cells expressing
Genetic evidence of a role for Lck in T-cell receptor function independent or downstream of ZAP-70/Syk protein tyrosine kinases
Wong, J., et al
Molecular and cellular biology, 18, 2855-2866 (1998)
E M Schaeffer et al.
Science (New York, N.Y.), 284(5414), 638-641 (1999-04-24)
T cell receptor (TCR) signaling requires activation of Zap-70 and Src family tyrosine kinases, but requirements for other tyrosine kinases are less clear. Combined deletion in mice of two Tec kinases, Rlk and Itk, caused marked defects in TCR responses
Nir Shani et al.
Blood, 113(15), 3530-3541 (2008-10-22)
The default pathway of cell-surface T-cell receptor (TCR) complex formation, and the subsequent transport to the membrane, is thought to entail endoplasmic reticulum (ER) localization followed by proteasome degradation of the unassembled chains. We show herein an alternative pathway: short
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-253 | 04053252330896 |
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