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A2153

Bovine Serum Albumin

Name: Bovine Serum Albumin
Brand: SIGMA

lyophilized powder, ≥96% (agarose gel electrophoresis)

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:

9048-46-8

UNSPSC Code:

12352202

EC Number:

232-936-2

NACRES:

NA.27

MDL number:

MFCD00130384

eCl@ss:

32160409

Biological source:

bovine

Assay:

≥96% (agarose gel electrophoresis)

Form:

lyophilized powder

Technique(s):

ELISA: suitable, blood typing: suitable, microbiological culture: suitable

Impurities:

≤6.0% Loss on Drying

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Properties

biological source

bovine

Quality Level

200

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable, blood typing: suitable, microbiological culture: suitable

impurities

≤6.0% Loss on Drying

loss

≤6%

pH

6.5-7.5 (1% in 0.15 M sodium chloride), 7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

BT Virus, none detected, VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Description

General description

Bovine Serum Albumin (BSA) is a globular, non-glycosylated α-helical plasma protein produced in the bovine liver. It consists of three domains (I, II, and III), each with two sub-domains (A and B), arranged in a heart-shaped structure.

Application

Bovine serum albumin has been used-

along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis
as a component of modified Krebs-Ringer buffer (dmKRBT)
in gel shift analysis
as a blocking agent in immunocytochemistry, immunohistochemistry,(4) immunofluorescence protocol and sandwich enzyme-linked immunosorbent assay (ELISA)
as a component of phosphate buffer saline (PBS) solution to resuspend cells for analysis during flow cytometry
as a protein standard to quantify proteins in tissue samples via Bradford’s method
to measure the enzyme activity of 17β HSD enzyme

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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