P3375
Papain from papaya latex
crude powder, 1.5-10 units/mg solid
Synonym(s):
Papainase
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About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42030311
EC Number:
232-627-2
NACRES:
NA.54
MDL number:
Product Name
Papain from papaya latex, crude powder, 1.5-10 units/mg solid
form
crude powder
specific activity
1.5-10 units/mg solid
mol wt
23.4 kDa
storage temp.
2-8°C
Quality Level
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Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Analysis Note
Not standardized with lactose or other adulterants.
Application
Papain from papaya latex has been used:
- in the enzyme cocktail to evaluate the efficacy of B complex vitamers liberation from nutritional yeast powder
- in the enzyme mixture for the digestion of bovine milk samples for the analysis of three vitamin B1 molecules
- as a biocatalyst for the hydrolysis of (R,S)-naproxen 2,2,2-trifluoroethyl ester in water-saturated solvents
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P3375, papain crude powder, has been used to digest fresh cartilage of dogsfish in order to isolate chondroitin sulfate.
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Other Notes
One unit will hydrolyze one micromole of N-alpha-benzoyl-l-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C.
View more information on papain at www.sigma-aldrich.com/enzymeexplorer.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Comparison of different extraction methods for simultaneous determination of B complex vitamins in nutritional yeast using LC/MS-TOF and stable isotope dilution assay
Halvin K, et al.
Analytical and Bioanalytical Chemistry, 405(4), 1213-1222 (2013)
Carica papaya lipase: a novel biocatalyst for the enantioselective hydrolysis of (R, S)-naproxen 2, 2, 2-trifluoroethyl ester
Chen CC and Tsai SW
Enzyme and Microbial Technology, 36(1), 127-132 (2005)
Determination of the native forms of vitamin B1 in bovine milk using a fast and simplified UHPLC method
Schmidt A, et al.
Food Chemistry, 229, 452-457 (2017)
Jocelyn L Y Tang et al.
Developmental cell, 57(9), 1193-1207 (2022-04-29)
Temporal patterning of neural progenitors is an evolutionarily conserved strategy for generating neuronal diversity. Type II neural stem cells in the Drosophila central brain produce transit-amplifying intermediate neural progenitors (INPs) that exhibit temporal patterning. However, the known temporal factors cannot
David L Burrows et al.
Journal of analytical toxicology, 29(5), 275-295 (2005-08-18)
The estimated number of employees in the United Stated screened annually for illicit drugs is approximately 20 million, with marijuana being the most frequently abused drug. Urine adulterants provide an opportunity for illicit drug users to obtain a false-negative result
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