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MAB5460-I-100UL Anti-Nkx2.1 Antibody, clone 8G7-G3-1

Anti-Nkx2.1, clone 8G7-G3-1, Cat. No. MAB5460-I, is a mouse monoclonal antibody that detects Homeobox protein Nkx-2.1 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

MAB5460-I-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB5460-I-100UL
      DescriptionAnti-Nkx2.1 Antibody, clone 8G7-G3-1
      Alternate Names
      • Homeobox protein Nkx-2.1
      • Thyroid nuclear factor 1
      • Thyroid transcription factor 1
      • TTF-1
      Background InformationHomeobox protein Nkx-2.1 (UniProt: P23441; also known as Thyroid nuclear factor 1, Thyroid transcription factor 1, TTF-1) is encoded by the Nkx2 (also known as Nkx-2.1, Titf1, Ttf-1, Ttf1) gene (Gene ID: 25628) in rat. Nkx-2.1 is a nuclear protein that is involved in the development and function of the brain, lungs, and thyroid gland. It serves as a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. It recognizes and binds to specific DNA sequences via a 60-amino acid DNA-binding domain called homeodomain. It is reported to be crucial in the maintenance of the thyroid differentiation phenotype. Its expression is restricted to regions of the developing brain within the diencephalon, in parts of the hypothalamus and neurohypophysis, and in the telencephalon. Its expression is shown to oscillate in a diurnal and melatonin-dependent manner in the preoptic area region in the hypothalamus, with maximal expression attained during the dark phase of the light/dark cycle. Nkx-2.1 is a highly selective marker for pulmonary adenocarcinoma and is a highly sensitive and specific immunomarker for distinguishing metastatic pulmonary from extrapulmonary adenocarcinoma.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibodyIgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Nkx2.1, clone 8G7-G3-1, Cat. No. MAB5460-I, is a mouse monoclonal antibody that detects Homeobox protein Nkx-2.1 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nkx-2.1 in A549 cells.

      Immunocytochemistry Analysis: A representative lot detected Nkx-2.1 in Immunocytochemistry applications (Liu, Y., et. al. (2013). Nat Protoc. 8(9):1670-9).

      Immunofluorescence Analysis: A representative lot detected Nkx-2.1 in Immunofluorescence applications (Jiajie, T., et.al. (2018). FEBS Open Bio . 8(4):502-512).

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Nkx-2.1 in rat lung tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenRecombinant rat Nkx2.1.
      EpitopeUnknown
      Clone8G7-G3-1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 8G7-G3-1 is a mouse monoclonal antibody that detects Homeobox protein Nkx-2.1.
      IsotypeIgG1
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Nkx2
      • Nkx-2.1
      • Titf1
      • Ttf-1
      • Ttf1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~44 kDa observed; 38.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat Lung tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nkx2.1 in Rat Lung tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB5460-I-100UL 04065265398091