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05-636-AF488 Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate Antibody

This Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags Gene Symbol: H2AFX, H2AX UniProt Number: P16104
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05-636-AF488
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number05-636-AF488
      DescriptionAnti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate Antibody
      Alternate Names
      • Histone H2AX
      • H2a/x
      • Histone H2A.X
      Background InformationHistone H2A.X is a variant of histone H2A, and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins.
      References
      Product Information
      FormatAlexaFluor®488
      HS Code3002 15 90
      PresentationPurified mouse conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% Sodium Azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
      Key Applications
      • Immunocytochemistry
      Biological Information
      ImmunogenLinear peptide corresponding to phospho-Histone H2A.X (Ser139).
      CloneJBW301
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Vertebrates based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • H2AFX
      • H2AX
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight17 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in untreated (negative control) and Staurosporin treated HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H2A.X (Ser139) in Staurosporin treated HeLa cells, and not in untreated HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-636-AF488 04053252971907

      Documentation

      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho Histone H2A.X (Ser139), -2659577 2659577
      Anti-phospho Histone H2A.X (Ser139), -2698465 2698465
      Anti-phospho Histone H2A.X (Ser139), -2710199 2710199
      Anti-phospho Histone H2A.X (Ser139), -2736393 2736393
      Anti-phospho Histone H2A.X (Ser139), -2795747 2795747
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, 2471006
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® -2673162 2673162
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate 2982363
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate 3033074
      Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate 3037397
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies