Filtration Discs
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Overview
Specifications
Ordering Information
Documentation
References
| Reference overview | Application |
|---|---|
| MicroRNA expression detected by oligonucleotide microarrays: System establishment and expression profiling in human tissues Omer Barad, Eti Meiri, Amir Avniel, Ranit Aharonov, Adi Barzilai, Isaac Bentwich, Uri Einav, Shlomit Gilad, Patrick Hurban, Yael Karov, Edward K. Lobenhofer, Eilon Sharon, Yoel M. Shiboleth, Marat Shtutman, Zvi Bentwich and Paz Einat Genome Research 14:2486-2494, 2004 2004 | |
| Highly selective membranes in protein ultrafiltration Feins M, Sirkar KK,Biotechnol Bioeng. 2004 Jun 20;86(6):603-11 Biotechnol Bioeng. 2004 Jun 20;86(6):603-11 2004 | Protein Purification |
| Electrokinetic characterisation of ultrafiltration membranes by streaming potential, electroviscous effect, and salt retention Huisman IH (REPRINT) ; Pradanos P; Hernandez A Journal of membrane science 2000, V178, N1-2, p55-64 2000 2000 | |
| Effect of Solution pH on Protein Transport Through Ultrafiltration Membranes Douglas B. Burns and Andrew L. Zydney Biotechnol Bioeng 64: 27-37, 1999. 1999 | |
| Integrated process for the removal of emulsified oils from effluents in the steel industry Benito JM; Rios G; Gutierrez B; Pazos C; Coca J (REPRINT) Separation Science and Technology 1999, V34, N15, P3031-3043 SN- 0149-6395 1999 | Industrial QC |
| General approach for the development of high-performance liquid chromatography methods for biosurfactant analysis and purification Lin, S.-C. Chen, Y.-C. Lin, Y.-M. J. Chromatogr., A ; Journal of Chromatography, A v. 825 pg 149-159 6 Nov 1998 1998 | |
| Filtration 15 nm: Aspects Techniques Chtourou S,Sang thrombose vaisseaux, 21-27, 1998 Sang thrombose vaisseaux, 21-27, 1998 1998 | |
| Distribution of trace elements associated with dissolved compounds (<0.45 .mu.m-1 nm) in freshwater using coupled (frontal cascade) ultrafiltration and chromatographic separations Pham, M. K. Garnier, J.-M. Environmental Science and Technology, v32, pg. 440-449, 15 Feb. 1998 1998 | |
| Acrylamide production in an ultrafiltration-membrane bioreactor using cells of Brevibacterium imperialis CBS 489-74 Cantarella M (reprint) ; Spera A; Cantarella L; Alfani F ; Journal of membrane science, v147, N2, p279-290 1998 Journal of membrane science, v147, N2, p279-290 1998 1998 | Enzyme Assays |
| In vitro and in vivo grafting of xeno pig fetal liver fragments using ultrafiltration membrane Kanai N.; Morita N.; Munkhbat B.; Gansuvd B.; Hagihara M.; Nagamachi Y.;Tsuji K. Cell Transplantation SO- 7/4 (417-420) 1998 1998 | Cell Culture |
FAQ
| Question | Answer |
|---|---|
| Is the Ultracel YM membrane autoclavable? | Yes, the Ultracel YM membrane can be autoclaved, submerged in water, at 121 degrees C. |
| Which UF membrane do I want, Biomax or Ultracel? | Biomax is recommended for applications where very harsh pH conditions are required for processing or cleaning. Ultracel is recommended where harsh pH conditions are not needed and especially when protein loading is low (<20-g/m sq.) or the feedstock is highly fouling. Biomax is currently the premier UF membrane being used in Life Science applications, primarily due to its combination of low protein binding (i.e., high yield) and speed. Ultracel, Millipore’s new composite regenerated cellulose offering, is the lowest protein binding UF membrane available, making it ideal for dilute protein solutions where binding may be a significant factor in yield. For important applications, such as pilot trials prior to scale-up, it is recommended to conduct a trial with several pore sizes from each membrane family in order to determine which best meets the application criteria. |
| When doing diafiltration, how many initial volumes would it take to remove the salt? | It takes three initial volumes to remove 95% of the salt and five initial volumes to remove 99.9% of the salt. |
| What molecular weight cut-off should I select for proteins of different sizes? | As a rule, when using Ultracel membranes it is best to select a membrane 2-3 times smaller than the solute to be retained by the membrane. In the case of the Biomax membranes 4-5 times smaller than the NMWL is suggested. For more information on choosing the appropriate membrane cutt off: http://www.millipore.com/publications.nsf/docs/RF036 |
| What is the best way to store disc membranes? | Solutions of 10% ethanol in water or 0.1% azide in water will preserve the membrane. For long-term storage, refrigeration is recommended. |
| What is the pH range for YM membrane? | The pH range is 3-13. |
| My recovery is low. Why? | 1. What concentration are you using? a. If it is protein and <10ug/ml, the protein may be binding to the plastic (not the membrane). We suggest passivation (See passivation protocol). b. If it is DNA and >100ug/ml, most likely the DNA is precipitating out. There is no solution except use less DNA. 2. Are you using a detergent? a. If yes, and it is greater than the critical micelle concentration, it will not pass through the membrane pores. Suggest diluting out the sample (See detergent study). |
| What is the temperature limit for the Ultracel YM membrane? | The temperature limit for the Ultracel YM membrane is 121 degrees C. |
| What is the temperature limit for the Biomax membrane? | The temperature limit for the Biomax membrane is 50 degrees C. |
| What is the average thickness of the Ultracel YM membrane? | The average thichness of the Ultracel YM membrane is 8 mil. |
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Categories
| Reagents, Chemicals and Labware > Lab Filtration > Ultrafiltration Membrane Discs |