PharmPrep™ P Sorbent

High Performance Spherical Silica for Efficient Peptide Purification

PharmPrep™ P is a spherical, porous silica sorbent available in two particle sizes: 10 and 20 μm for preparative liquid chromatography (LC). With a pore diameter of 100Å (10 nm), these sorbents fit perfectly into the polishing step of small peptides like insulin, and other biopharmaceutical and active pharmaceutical ingredients (APIs) like antibiotics and hormones.

PharmPrep™ P sorbents are characterized by:

• Uniform and homogenous silica gel matrix with excellent batch-to-batch reproducibility over many column packings
• Narrow particle size distribution for high performance and high productivity in small peptide purification processes
• Reproducible specific surface area and pore size distribution
• Low back pressure
• Enhanced mechanical stability for long sorbent life-time

Superior polishing of insulin

PharmPrep™ P sorbent demonstrates a perfect separation of insulin and the main impurities desamido-insulin. Using PharmPrep™ P sorbent in your polishing step, an outstanding insulin purity of 99.8% can be achieved.

PharmPrep™ P 100 RP-18e, 10 μm sorbent chromatographic conditions for polishing of crude human insulin (90% pure) Column Size 250 x 4.6 mm Mobile phase A: 0.1 M NH4H2PO4 pH 7.3 B: 0.1 M NH4H2PO4 pH 7.3/ACN 5/5 (Gradient mode 65% A–30% A 100 min) Flow rate 1.325 mL/min Temp. 25 °C Detection UV 214 nm Injection volume 100 μL Sample 18 mg/mL crude human Insulin rec. dissolved in water +0.1% TFA

Three types of PharmPrep™ P Sorbents are available:

PharmPrep™ P: for Normal Phase Chromatography

• PharmPrep™ P Si 100 10µm 
• PharmPrep™ P Si 100 20µm 

PharmPrep™ P RP: for Reversed Phase Chromatography

• PharmPrep™ P RP 18e 10µm
• PharmPrep™ P RP 18e 20µm 
• PharmPrep™ P RP 8e 10µm 

The PharmPrep™ P RP sorbents are also available in our prepacked stainless steel columns:

• PharmPrep™ P RP 18e 10µm 
• PharmPrep™ P RP 18e 20µm
• PharmPrep™ P RP 8e 10µm