N2415
Nucleoside Phosphorylase bacterial
recombinant, expressed in E. coli, ≥10 units/mg protein
동의어(들):
PNP, Purine nucleoside phosphorylase, Purine nucleoside:orthophosphate ribosyltransferase
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제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
NACRES:
NA.54
UNSPSC Code:
12352204
EC Number:
232-857-3
MDL number:
Specific activity:
≥10 units/mg protein
Assay:
≥80% protein basis (biuret)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
assay
≥80% protein basis (biuret)
form
lyophilized powder
specific activity
≥10 units/mg protein
sequence note
MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVRGMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLRDVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLFYSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAERQTTFNDMIKIALESVLLGDKE
UniProt accession no.
storage temp.
−20°C
SMILES string
[n]2(c3ncncc3nc2)[C@@H]1O[C@@H]([C@H]([C@H]1O)O)CO
InChI
1S/C10H12N4O4/c15-2-6-7(16)8(17)10(18-6)14-4-13-5-1-11-3-12-9(5)14/h1,3-4,6-8,10,15-17H,2H2/t6-,7-,8-,10-/m1/s1
InChI key
MRWXACSTFXYYMV-FDDDBJFASA-N
Gene Information
Escherichia coli ... deoD(945654)
Application
Nucleoside Phosphorylase bacterial has been used in reaction buffer and buffer-exchanged to remove residual phosphate.
Bacterial nucleoside phosphorylase has been used in a study that identified and characterized two adenosine phosphorylase activities in Mycobacterium smegmatis. Bacterial nucleoside phosphorylase has also been used in a study to investigate the inhibitionof pyrimidine and purine nucleoside phosphorylases by a 3,5-dichlorobenzoyl-substituted 2-deoxy-D-ribose-1-phosphate derivative.
Nucleoside phosphorylase is used in coupled enzyme systems to measure protein dephosphorylation.
Biochem/physiol Actions
Nucleoside Phosphorylase/purine nucleoside phosphorylase (PNP), an enzyme participates in the purine salvage pathway. It also helps in the reversible cleavage of inosine to hypoxanthine and guanosine to guanine. PNP deficiency results in neurologic manifestations.
Catalyzes the following reaction:purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate
Preparation Note
This is a salt-free and animal component-free enzyme preparation. This enzyme should be solubilized in a 10mM KPO4, pH 7.4 solution.
Other Notes
One unit will cause the phosphorolysis of 1.0 micromole of inosine to hypoxanthine andribose-1-phosphate per min at pH 7.4 at 25 deg C.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable