|Description||Guava Mitochondrial Depolarization Kit for Flow Cytometry|
|Overview||Guava Mitochondrial Depolarization Assay
for detection of early apoptosis
There is growing interest within the scientific community to evaluate changes in the mitochondrial membrane potential and further define its role in initiating apoptosis, cell cycle, and other cellular processes. Automated single-cell analysis is the technique of choice due to its sensitivity and high reproducibility.
Guava Technologies has optimized an assay format for 96-well plates that combines the sensitivity of a single-cell based method with the throughput and ease-of-use of a microplate reader. The Guava® Mitochondrial Depolarization Assay for the Guava EasyCyte™ Plus System allows you to monitor the loss of mitochondrial membrane potential through the use of the fluorescent cationic dye, JC-1. An optional dead cell dye, 7-AAD, is also included for discerning apoptotic cells from dead cells.
Distinguishes healthy, early apoptotic, and dead cells
In healthy, non-apoptotic cells, the JC-1 dye enters the mitochondrial matrix, accumulates as aggregates and stains the mitochondria bright red. In apoptotic cells, the mitochondrial membrane potential collapses, and the JC-1 enters the cytoplasm as a monomeric form where it fluoresces green. Results are automatically calculated and presented in a statistical format that is easy to interpret, with total cell counts and percentages for those cell populations that are either healthy, apoptotic, or dead. For use with EasyCyte Mini, EasyCyte Plus. Click here for more information regarding Instrument Compatibility
|Application||Guava Mitochondrial Depolarization Assay for detection of early apoptosis.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Material Size||100 Tests|
Guava Mitochondrial Depolarization Kit for Flow Cytometry SDB
|Guava ® MitoPotential Kit - 100 Tests - 12-0593||12-0593|
|Guava ® MitoPotential Kit - 100 Tests - 13-0533||13-0533|