4500-0250 Guava Mitochondrial Depolarization Kit for Flow Cytometry

100 Tests  
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      Replacement Information
      Catalogue Number4500-0250
      Trade Name
      • Guava
      DescriptionGuava Mitochondrial Depolarization Kit for Flow Cytometry
      OverviewGuava Mitochondrial Depolarization Assay
      for detection of early apoptosis
      There is growing interest within the scientific community to evaluate changes in the mitochondrial membrane potential and further define its role in initiating apoptosis, cell cycle, and other cellular processes. Automated single-cell analysis is the technique of choice due to its sensitivity and high reproducibility.
      Microplate protocol
      Guava Technologies has optimized an assay format for 96-well plates that combines the sensitivity of a single-cell based method with the throughput and ease-of-use of a microplate reader. The Guava® Mitochondrial Depolarization Assay for the Guava EasyCyte™ Plus System allows you to monitor the loss of mitochondrial membrane potential through the use of the fluorescent cationic dye, JC-1. An optional dead cell dye, 7-AAD, is also included for discerning apoptotic cells from dead cells.
      Distinguishes healthy, early apoptotic, and dead cells
      In healthy, non-apoptotic cells, the JC-1 dye enters the mitochondrial matrix, accumulates as aggregates and stains the mitochondria bright red. In apoptotic cells, the mitochondrial membrane potential collapses, and the JC-1 enters the cytoplasm as a monomeric form where it fluoresces green. Results are automatically calculated and presented in a statistical format that is easy to interpret, with total cell counts and percentages for those cell populations that are either healthy, apoptotic, or dead. For use with EasyCyte Mini, EasyCyte Plus. Click here for more information regarding Instrument Compatibility
      Product Information
      • JC-1
      • 7-AAD
      • Detailed Protocol
      Instrument compatibility
      • EasyCyte
      • EasyCyte Plus
      • EasyCyte Mini
      • EasyCyte 8HT
      Quality LevelMQ300
      ApplicationGuava Mitochondrial Depolarization Assay for detection of early apoptosis.
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size100 Tests
      Transport Information
      Supplemental Information

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      Life Science Research > Cell Analysis > guava easyCyte Flow Cytometers > Flow Cytometry Kits & Reagents