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Stericup® Filtereinheiten

Das Stericup Vakuum-Filtrationssystem kombiniert eine Steritop Filtereinheit mit einer Filtratsammelflasche für die Filtration und Lagerung von 150 ml bis 1.000 ml.

Das Stericup Vakuum-Filtrationssystem kombiniert eine Steritop Filtereinheit mit einer Filtratsammelflasche für die Filtration und Lagerung von 150 ml bis 1.000 ml.

Die konische Form der Auffangflasche verbessert die Stabilität der Einheit während der Filtration und erleichtert die Handhabung. Die kompakte, schlanke Form ist außerdem ideal für den Einsatz unter Laminar-Flow Bedingungen.

Der etwas gewölbte Boden der Flaschen ermöglicht das Stapeln der verschlossenen Flaschen aufeinander.

Überblick

Spezifikationen

Bestellinformationen

Einheiten für die Sterilfiltration wässriger Lösungen und Mykoplasma-Abscheidung. 0,1 µm Millipore Express (PES)-Membran mit hoher Fließgeschwindigkeit und geringer Proteinbin dung. Lieferung: sterilSortierung & Filter aufheben Show Filter
BestellnummerBeschreibungTrichtervolumenAuffanggefäßSt./Pkg.
SCVPU02REStericup-VP, 0,10 µm, Polyethersulfon, 250 ml, gamma-sterilisiert 250 mL 250-ml-Flasche mit Verschluss 12 Preis & Verfügbarkeit

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Einheiten für die Sterilfiltration wässriger Lösungen. 0,22 µm Durapore (PVDF)-Membran mit extrem geringer Proteinbindung. Lieferung: sterilSortierung & Filter aufheben Show Filter
BestellnummerBeschreibungTrichtervolumenAuffanggefäßSt./Pkg.
SCGVU05REStericup-GV, 0,22 µm, PVDF, 500 ml, -sterilisiert 500 mL 500-ml-Flasche mit Verschluss 12 Preis & Verfügbarkeit

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Documentation

FAQ

FrageAntwort
When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening?Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line.
What is the typical holdup volume for Stericup.The hold up volume is about 6 ml. If you give the unit a slight shake, you can reduce the holdup volume by about 60% to 2.4 ml. Solutions containing protein have a slightly higher holdup volume, about 7 ml, again reduced to less than half by a slight shake.
What is the diameter of the membrane in the Stericup and Steritop units?All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area.
Why use a vacuum driven filter rather than a pressure driven filter?Unlike positive pressure filtration, vacuum driven filtration requires only a vacuum source. It does not require pressure vessels or peristaltic tubing pumps. Stericup and Steritop units are pre-sterilized and disposable making setup and cleanup easy!
Should I filter my acrylamide solution prior to gel casting?Yes, filtering your acrylamide solution before adding the polymerization activator ammonium persulfate and TEMED will accomplish two things.
1. It will eliminate particulate matter that may interfere with base calling when sequencing or that may create "hot" spots in the gel, and
2. It will aid in degassing, which will promote efficient polymerization of the gel.

Products appropriate for this application would be Stericup, Steritop or Steriflip with express membrane.
It sounds like air is leaking into my Stericup, what should I do?First make sure that the top is securely fastened to the base. Discontinue use if there appears to be any cracks on the apparatus.
What Stericup would you recommend to remove mycoplasma?The Stericup VP ontains an 0.1um Express membrane. The VP membrane has been validated for the removal of mycoplasma.
Which pre-filter should I be using with my Stericup or Steritop unit?You should use an AP20 75mm pre-filter (AP2007500). To help keep the filter from floating in the solution to be filtered, insert the pre-filter under the pre-filter tab inside the Stericup or Steritop.
What is the expiration date on Stericup and Steritop units?The expiration date, which specifies product sterility, for the Stericup and Steritop products is three years from the date of manufacture.
What type of flow rates can I expect with the Stericups and Steritops?For typical non -serum tissue culture media you can expect flow rates from 1-1.5 lpm.

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Kategorien

Life Science Research > Cell Culture and Systems > Sterile Filtration Devices