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MABN2287 Anti-APP Antibody, clone 1D1

Anti-APP, clone 1D1, Cat. No. MABN2287, is a highly specific rat monoclonal antibody that targets Amyloid beta A4 protein and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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MABN2287
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2287
      DescriptionAnti-APP Antibody, clone 1D1
      Alternate Names
      • Amyloid beta A4 protein
      • ABPP
      • APPI
      • Alzheimer disease amyloid protein
      • Amyloid precursor protein
      • Beta-amyloid precursor protein
      • Cerebral vascular amyloid peptide
      • CVAP
      • PreA4
      • Protease nexin-II
      • PN-II
      Background InformationAmyloid beta A4 protein (UniProt: P05067; also known as ABPP, APPI, APP, Alzheimer disease amyloid protein, Amyloid precursor protein, Beta-amyloid precursor protein, Cerebral vascular amyloid peptide, CVAP, PreA4, Protease nexin-II, PN-II) is encoded by the APP (also known as A4, AD1) gene (Gene ID: 351) in human. APP undergoes extensive post-translational modification including glycosylation, phosphorylation, and tyrosine sulfation, as well as many types of proteolytic processing to generate peptide fragments. APP is proteolytically processed under normal cellular conditions by alpha-secretase or beta-secretase to generate and release soluble APP peptides, S-APP-alpha and S-APP-beta, and the retention of corresponding membrane-anchored C-terminal fragments, C80, C83 and C99. Subsequent processing of C80 and C83 by gamma-secretase yields P3 peptides. In Alzheimer s disease processing of C99 generates amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42) that form amyloid plaques. Beta-amyloid peptides are lipophilic metal chelators with metal-reducing activity. They bind transient metals such as copper, zinc and iron. APP can also be cleaved by caspases during neuronal apoptosis. Cleavage at Asp-739 by either caspase-6, -8 or -9 results in the production of the neurotoxic C31 peptide and the increased production of beta-amyloid peptides. In addition to its obvious role in Alzheimer's disease, the most-substantiated role for APP is in synaptic formation and repair. Its expression is upregulated during neuronal differentiation and after neural injury. Clone 1D1 is shown to bind to the N-terminus of APP and detects both the soluble full length and secreted hAPP and can also detect transgenic APP expression in APP-transgenic animal models.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-APP, clone 1D1, Cat. No. MABN2287, is a highly specific rat monoclonal antibody that targets Amyloid beta A4 protein and has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesNote: For the Western blotting application use of nitocellulose is highly recommended and the samples must be boiled in non-reduing laemmli buffer. Please note that clone 1D1 recognizes APP only in its quasi-native folded form.

      Immunoprecipitation Analysis: A representative lot detected APP in Immunoprecipitation applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

      Western Blotting Analysis: A representative lot detected APP in WT, but not in APP knockdown HEK293T cells (Courtesy of Dr. med. Peer-Hendrik Kuhn, Ph.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany).

      Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected APP in HEK293 cell line.

      Immunocytochemistry Analysis: A representative lot detected APP in Immunocytochemistry applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

      Immunocytochemistry Analysis: A 1:10 dilution from a representative lot detected APP in HEK293T cells, but not in cells lentivirally transduced with APP shRNA-1 or APP shRNA-2, both coexpressing GFP as a reporter (Courtesy of Dr. med. Peer-Hendrik Kuhn, Ph.D., Institut fur Allgemeine Pathologie und Pathologische Anatomie, Technische Universität München, Munich, Germany).

      Immunohistochemistry Analysis: A representative lot detected APP in Immunohistochemistry applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected APP in human cerebral cortex and human Alzheimer's brain tissues.

      ELISA Analysis: A representative lot detected APP in ELISA applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

      Western Blotting Analysis: A representative lot detected APP in Western Blotting applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).

      Flow Cytometry Analysis: A representative lot detected APP in Flow Cytometry applications (Hofling, C., et. al. (2016). Aging Cell. 15(5):953-63).
      Biological Information
      ImmunogenStrepII-tagged human recombinant ectodomain region of the neuronal isoform of hAPP695 lacking the KPI domain.
      Clone1D1
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 1D1 specifically detects human amyloid precursor protein (APP) and does not display reactivity with other species. It targets an epitope within the ectodomain region of APP and does not react with A beta peptide.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APP
      • A4
      • AD1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~100 kDa observed; 86.94 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected APP in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2287 04054839410529

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