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MABF1479-100UG Anti-CD120b/TNFR2 Antibody, clone TR75-89

Anti-CD120b/TNFR2, clone TR75-89, Cat. No. MABF1479, is an Armenian hamster monoclonal antibody that detects CD120b/TNFR2 and is tested for use in Flow Cytometry, Immunoprecipitation, and Western Blotting.

MABF1479-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF1479-100UG
      DescriptionAnti-CD120b/TNFR2 Antibody, clone TR75-89
      Alternate Names
      • Tumor necrosis factor receptor superfamily member 1B
      • Tumor necrosis factor receptor 2
      • TNF-R2
      • Tumor necrosis factor receptor type II
      • TNF-RII
      • TNFR-II
      • p75
      • p80 TNF-alpha receptor
      Background InformationTumor necrosis factor receptor superfamily member 1B (UniProt: P25119; also known as Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, TNF-RII, TNFR-II, p75, p80 TNF-alpha receptor, CD120b) is encoded by the Tnfrsf1b (also known as Tnfr-2, Tnfr2) gene (Gene ID: 21938) in murine species. TNFR2 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 23-258), a transmembrane domain (aa 259-288), and a cytoplasmic domain (aa 289-474). It serves as a high-affinity receptor for TNF- . It displays about five-fold reduced affinity for lymphotoxin- . It acts as a co-stimulator for IL-2 Induction and T cell survival and enhances the responses of lymphocytes to TCR-mediated signaling. It plays a critical role during TCR/CD28-mediated stimulation toward IL-2 induction, clonal expansion, and survival. TNFR2 deficient T cells possess a profound defect in IL-2 production in response to TCR/CD28-mediated stimulation. It is also reported to induce proliferative responses in certain cells of hematopoietic origin and enhances expression of certain adhesion molecules such as ICAM-1. (Ref.: Chen, X., et al. (2010). J. Immunol. 185(1); 174-182; Kim, EY., and Teh, HS. (2004). J. Immunol. 173(7); 4500-4509; Sheehan, KCF., et al. (1995). J. Exp. Med. 181(2); 607-617).
      References
      Product Information
      FormatPurified
      PresentationPurified Armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CD120b/TNFR2, clone TR75-89, Cat. No. MABF1479, is an Armenian hamster monoclonal antibody that detects CD120b/TNFR2 and is tested for use in Flow Cytometry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunoprecipitation
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected CD120b/TNFR2 in Western Blotting applications (Sheehan, K.C., et al. (1995). J Exp Med. 181(2):607-17).

      Immunoprecipitation Analysis: A representative lot detected CD120b/TNFR2 in Immunoprecipitation applications (Pinckard, J.K., et al. (1997). J Immunol. 158(8):3869-73).

      Flow Cytometry Analysis: A representative lot detected CD120b/TNFR2 in Flow Cytometry applications (Kim, E.Y., et al. (2004). J Immunol. 173(7):4500-9).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenPurified extracellular domain of mouse Tumor necrosis factor receptor 2 (CD120b).
      EpitopeExtracellular domain
      CloneTR75-89
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostArmenian Hamster
      SpecificityClone TR75-89 is an Armenian hamster monoclonal antibody that detects Tumor necrosis factor receptor 2/CD120b. It targets an epitope within the extracellular domain.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tnfrsf1b
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight50.32 kDa calculated..
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in L929 cells.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected CD120b/TNFR2 in one million L929 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF1479-100UG 04065270938046