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MABT329-25UG Anti-Cytokeratin 8 Antibody, clone TROMA-1

Anti-Cytokeratin 8, clone TROMA-1 Antibody, Cat. No. MABT329-25UG is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence and Radioimmunoassay.

Anti-Cytokeratin 8 Antibody, clone TROMA-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Krt8, Krt2-8 UniProt Number: P11679
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MABT329-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IH(P), IP, IF, RIARPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT329-25UG
      DescriptionAnti-Cytokeratin 8 Antibody, clone TROMA-1
      Alternate Names
      • Keratin type II cytoskeletal 8
      • Cytokeratin endo A
      • Cytokeratin-8
      • CK-8
      • Keratin-8
      • K8
      • Type-II keratin Kb8
      Background InformationKeratin, type II cytoskeletal 8 (UniProt: P11679; also known as Cytokeratin endo A, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the Krt8 (also known as Krt2-8) gene (Gene ID: 16691) in murine species. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. Cytokeratins share a common structural organization: a central a-helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is a heterotetramer of two type I and two type II keratins. It is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. CK8 together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cytokeratin 8, clone TROMA-1 Antibody, Cat. No. MABT329-25UG is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Immunofluorescence and Radioimmunoassay.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Immunofluorescence
      • Radioimmunoassay
      Application NotesImmunocytochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunocytochemistry applications (O'Driscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Wang, X., et. al. (2004). J Cell Sci. 117(Pt 1):53-62).
      Biological Information
      ImmunogenIntermediate filaments extracted from TDM1 Trophoblastoma cells.
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone TROMA-1 detects cytokeratin-8 in human and murine cells.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Krt8
      • Krt2-8
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT329-25UG 04054839420993

      Documentation

      Anti-Cytokeratin 8 Antibody, clone TROMA-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cytokeratin 8 Antibody, clone TROMA-1 Certificates of Analysis

      TitleLot Number
      Anti-Cytokeratin 8, clone TROMA-1 - 3190886 3190886
      Anti-Cytokeratin 8, clone TROMA-1 - 3217771 3217771
      Anti-Cytokeratin 8, clone TROMA-1 - 3321792 3321792
      Anti-Cytokeratin 8, clone TROMA-1 - 3683145 3683145
      Anti-Cytokeratin 8, clone TROMA-1 - 3693113 3693113
      Anti-Cytokeratin 8, clone TROMA-1 - 3722605 3722605
      Anti-Cytokeratin 8, clone TROMA-1 - 3766675 3766675
      Anti-Cytokeratin 8, clone TROMA-1 - 3855211 3855211
      Anti-Cytokeratin 8, clone TROMA-1 - 3885483 3885483
      Anti-Cytokeratin 8, clone TROMA-1 - 3923040 3923040
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