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MABT335 Anti-Desmoglein-3, clone 5G11 Antibody

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MABT335
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT335
      ReplacesMABT470
      DescriptionAnti-Desmoglein-3, clone 5G11 Antibody
      Alternate Names
      • Desmoglein-3
      • 130 kDa pemphigus vulgaris antigen
      • PVA
      • Cadherin family member 6
      Background InformationDesmoglein-3 (DSG3) is a calcium binding membrane protein that is localized desmosome cellular junctions and interacts with plaque proteins and intermediate filaments at cell-cell adhesion points. Desmosomes are cell-cell junctions between epithelial, myocardial and other cells types. Strong intercellular adhesion is critical for tissues that experience mechanical stress, such as the skin and heart. Desmosomes provide adhesive strength to tissues by anchoring desmosomal cadherins of neighboring cells to the intermediate filament cytoskeleton. It is thought that raft membrane microdomains play a role in desmosome homeostasis by regulating the dynamics of desmosome assembly and disassembly. In human keratinocytes, Desmoglein-3 (DSG3) is raft associated and disruption of rafts prevents desmosome assembly. Desmoglein-3 is one of four sister proteins in the desmoglein family. Desmoglein-3 is also the autoantigen for Pemphigus vulgaris (PV) a lethal skin disease that is a result of autoantibodies against Desmoglein-3.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationThis Anti-Desmoglein-3, clone 5G11 Antibody is validated for use in Immunohistochemistry (Paraffin) for the detection of Desmoglein-3.
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A representative lot detected Isocitrate Desmoglein-3 in human scalp hair follicles and epidermal inclusions (Wu, H., et al. (2003). J Invest Dermatology. 120(6):1152-1157).
      Immunohistochemistry Analysis: A representative lot detected Isocitrate Desmoglein-3 in in human adult skin tissue (Wu, H., et al. (2013). New England Journal of Med. 343(1):31-35).
      Biological Information
      ImmunogenRecombinant protein corresponding to human Desmoglein-3.
      Clone5G11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DSG3
      • CDHF6
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight107 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human tonsil and human skin tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Desmoglein-3 in human tonsil and human skin tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT335 04053252983665

      Documentation

      Anti-Desmoglein-3, clone 5G11 Antibody Certificates of Analysis

      TitleLot Number
      Anti-Desmoglein-3, clone 5G11 -3166521 3166521
      Anti-Desmoglein-3, clone 5G11 - Q2443131 Q2443131
      Anti-Desmoglein-3, clone 5G11 - 3831418 3831418

      References

      Reference overviewPub Med ID
      Desmoglein isotype expression in the hair follicle and its cysts correlates with type of keratinization and degree of differentiation.
      Wu, Hong, et al.
      J. Invest. Dermatol., 120: 1052-7 (2003)  2003

      Show Abstract
      12787134 12787134
      Protection against pemphigus foliaceus by desmoglein 3 in neonates.
      Wu, H, et al.
      N. Engl. J. Med., 343: 31-5 (2000)  2000

      10882766 10882766