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MABC1745-100UG Anti-ErbB-3/HER3 Antibody, clone NG33

Anti-ErbB-3/HER3, clone NG33, Cat. No. MABC1745, is a mouse monoclonal antibody that detects ErbB-3/HER3 and is tested for use in ELISA, Flow Cytometry, Immunoprecipitation, and Inhibition Assay.

MABC1745-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1745-100UG
      DescriptionAnti-ErbB-3/HER3 Antibody, clone NG33
      Alternate Names
      • Receptor tyrosine-protein kinase erbB-3
      • EC:2.7.10.1
      • Proto-oncogene-like protein c-ErbB-3
      • Tyrosine kinase-type cell surface receptor HER3
      Background InformationReceptor tyrosine-protein kinase erbB-3 (UniProt: P21860; also known as EC:2.7.10.1, Proto-oncogene-like protein c-ErbB-3, Tyrosine kinase-type cell surface receptor HER3) is encoded by the ERBB3 (also known as HER3) gene (Gene ID: 2065) in human. ErbB3 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-643), a transmembrane domain (aa 644-664), and a cytoplasmic domain (aa 665-1342). Its extracellular domain is formed of two ligand-binding regions (L1 and L2) and two cysteine-rich regions (CR1 and CR2). In the absence of a ligand, the extracellular domain stays in a locked conformation and is stabilized by interactions between CR1 and CR2 regions that prevent its dimerization. Its tyrosine kinase domain is localized to amino acids 709-966 and shares homology with EGFR and ErbB2 kinase domain. However, the tyrosine kinase activity is impaired due to modified residues and its activation requires the formation of heterodimers with other EGFR family receptors. Known ligands for ErbB3 include neuregulins (NRG) and NRG1 and NRG2 appear to be the most active. Downstream signaling pathways activated include MAPK and PI3 Kinase pathways. ErbB3 has been implicated in cell division and survival and its expression is essential for cell proliferation in several tumor models and transformation of normal tissues. It has also been implicated in acquired resistance to EGFR-tyrosine kinase inhibitors. Clone NG33 is shown to compete with NRG for binding to ErbB3/HER3 and inhibits NRG-induced migration and proliferation of cancer cells. It is reported to induce faster, and more extensive receptor degradation compared to NRG. (Ref.: Kiavue, N., et al. (2020). Oncogene. 39(3); 487-502; Gaborit, N., et al. (2015). Proc. Natl. Acad. Sci. USA. 112(3); 839-844).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-ErbB-3/HER3, clone NG33, Cat. No. MABC1745, is a mouse monoclonal antibody that detects ErbB-3/HER3 and is tested for use in ELISA, Flow Cytometry, Immunoprecipitation, and Inhibition Assay.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      • Agonist or Inhibitor
      Application NotesTested Applications

      Inhibition Assay: A representative lot of this antibody inhibited neuregulin (NRG)-induced migration and proliferation of cancer cells. (Gaborit, N., et al. (2015). Proc Natl Acad Sci USA. 112(3):839-44).

      Flow Cytometry Analysis: A representative lot detected ErbB-3/HER3 in Flow Cytometry application (Gaborit, N., et al. (2015). Proc Natl Acad Sci USA. 112(3):839-44).

      ELISA Analysis: A representative lot detected ErbB-3/HER3 in ELISA applications (Gaborit, N., et al. (2015). Proc Natl Acad Sci USA. 112(3):839-44).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated ErbB-3/HER3 in Immunoprecipitation application (Gaborit, N., et al. (2015). Proc Natl Acad Sci USA. 112(3):839-44).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenA recombinant fragment corresponding to the extracellular domain of human ErbB-3/HER3 combined with the Fc domain of human IgG1.
      EpitopeExtracellular domain
      CloneNG33
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone NG33 is a mouse monoclonal antibody that detects ErbB-3/HER3. It targets an epitope within the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ERBB3
      Purification MethodPurified
      UniProt Number
      Molecular Weight148.1 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA with recombinant Human ErRB-3/HER3 protein.

      ELISA Analysis: Various dilutions of this antibody detected full-length recombinant Human ErbB-3/HER3 protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1745-100UG 04065270453686