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MABT12 Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4 detects level of Heparan Sulfate Proteoglycan (Perlecan) & has been published & validated for use in IH.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: HSPG2, SJA, perlecan, SJS, HSPG, PRCAN, Perlecan, SJS1, PLC UniProt Number: P98160
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MABT12
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIHC, ICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT12
      DescriptionAnti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4
      Alternate Names
      • heparan sulfate proteoglycan 2 (domain V region)
      • perlecan proteoglycan
      • basement membrane-specific heparan sulfate proteoglycan core protein
      • HSPG
      • PLC
      Background InformationPerlecan is one of the extracellular matrix forms of heparan sulfate proteoglycans (HSPGs). It is a large multidomain and binds to and cross-links many extracellular matrix (ECM) components and cell-surface molecules. Perlecan is synthesized by vascular endothelial and smooth muscle cells and deposited in the extracellular matrix within all mammalian tissues. HSPGs differ in their protein cores that are thought to determine the location of HSPG in either the cell membrane (syndecan and glypican) or the extracellular matrix (perlecan and dystroglycan). HSPGs found in the brain are dystroglycan, N-syndecan glypican, and perlecan. The composition of HS isolated from brain differs significantly from that of other organs and is developmentally regulated. Thus, differences in protein cores, disaccharide composition, the extent and position of sulfation, and the number of GAG chains diversify chemical structure and functions of HSPGs. The postulated functions of HSPG include cell proliferation, differentiation, adhesion, migration, and morphogenesis.
      References
      Product Information
      FormatPurified
      Control
      • Fresh-frozen normal human tonsil tissue
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4 detects level of Heparan Sulfate Proteoglycan (Perlecan) & has been published & validated for use in IH.
      Key Applications
      • Immunohistochemistry
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. (courtesy of Whitelock, J. Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia)

      Western Blot Analysis: A previous lot was used by an independent laboratory on a 3-8% Tris-acetate gel in which the band appears above the 460 kDa marker. (courtesy of Whitelock, J. Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia)
      Biological Information
      ImmunogenFull length recombinant protein corresponding to human Perlecan.
      Clone5D7-2E4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and Transthyretin, etc. and plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and Tardive dyskinesia.
      Gene Symbol
      • HSPG2
      • SJA
      • perlecan
      • SJS
      • HSPG
      • PRCAN
      • Perlecan
      • SJS1
      • PLC
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: This protein is an integral component of basement membranes. It is responsible for the fixed negative electrostatic charge and is involved in the charge-selective ultrafiltration properties. It serves as an attachment substrate for cells.

      SIZE: 4391 amino acids; 468825 Da

      SUBUNIT: Purified perlecan has a strong tendency to aggregate in dimers or stellate structures. It interacts with other basement membrane components such as laminin, prolargin and collagen type IV. Interacts with COL13A1, FGFBP1 and VWA1.

      SUBCELLULAR LOCATION: Secreted, extracellular space.

      TISSUE SPECIFICITY: Found in the basement membranes.

      PTM: N- and O-glycosylated; contains three heparan sulfate chains.

      DISEASE: Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses. & Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.

      SIMILARITY: Contains 4 EGF-like domains. & Contains 22 Ig-like C2-type (immunoglobulin-like) domains. & Contains 11 laminin EGF-like domains. & Contains 3 laminin G-like domains. & Contains 3 laminin IV type A domains. & Contains 4 LDL-receptor class A domains. & Contains 1 SEA domain.
      Molecular Weight 469 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in fresh-frozen normal human tonsil tissue.

      Immunohistochemistry Analysis: 1:300 dilution of the antibody detected Perlecan in fresh-frozen normal human tonsil tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT12 04053252413254

      Documentation

      Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone 5D7-2E4 Certificates of Analysis

      TitleLot Number
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 2148265 2148265
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 2352421 2352421
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 2277889 2277889
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 3041057 3041057
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 3232376 3232376
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 3862688 3862688
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 3949246 3949246
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 4037461 4037461
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - 4331585 4331585
      Anti-Heparan Sulfate Proteoglycan (Perlecan), clone 5D7-2E4 - NRG1791891 NRG1791891
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies