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MABF2293-25UG Anti-IL-10 Antibody, clone 2H6

Anti-IL-10, clone 2H6, Cat. No. MABF2293, is a mouse monoclonal antibody that detects human Interleukin-10 and has been tested for use in Affinity Binding Assay and Immunohistochemistry (Paraffin).

MABF2293-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2293-25UG
      DescriptionAnti-IL-10 Antibody, clone 2H6
      Alternate Names
      • Interleukin-10
      • Cytokine synthesis inhibitory factor
      • CSIF
      Background InformationInterleukin-10 (UniProt: P22301; also known as IL-10, Cytokine synthesis inhibitory factor, CSIF) is encoded by the IL10 gene (Gene ID: 3586) in human. IL-10 is a homodimeric secreted anti-inflammatory cytokine produced by a variety of cells, including T-cells, macrophages, and mast cells. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature form. IL-10 acts on many cells of the immune system to limit excessive tissue disruption caused by inflammation. During infection it inhibits the activity of Th1 cells, NK cells, and macrophages, all of which are required for optimal pathogen clearance. It ameliorates the excessive Th1 and CD8+ T cell responses that are responsible for much of the immunopathology associated with infections. It also inhibits the release of pro-inflammatory cytokines. It is shown to control the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. IL-10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB, which leads to JAK1 and STAT2-mediated phosphorylation of STAT3 that in turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. (Ref.: Couper, KN., et al. (2008). J. Immunol. 180(9); 5771-5777).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-IL-10, clone 2H6, Cat. No. MABF2293, is a mouse monoclonal antibody that detects human Interleukin-10 and has been tested for use in Affinity Binding Assay and Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Affinity Binding Assay
      Application NotesAffinity binding assay: The binding of Anti-IL-10, clone 2H6, Cat. No. MABF2293, showed a KD value of 1.68 x 10-9, whereas binding to GST showed a KD of 7.22 x 10-3.
      Biological Information
      ImmunogenFull-length recombinant mature human IL-10 (without the signal peptide).
      Clone2H6
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2H6 is a mouse monoclonal antibody that specifically detects human interleukin-10 (IL-10).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IL10
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight20.52 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human tonsil tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected IL-10 in human tonsil tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2293-25UG 04061841235873