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ABN464 Anti-IRE1p Antibody

Detect Inositol-requiring protein 1 using this rabbit polyclonal antibody, Anti-IRE1p Antibody validated for use in western blotting, ICC & IHC.

Anti-IRE1p Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: ERN1, IREp1 UniProt Number: O75460
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ABN464
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, ICC, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN464
      DescriptionAnti-IRE1p Antibody
      Alternate Names
      • Endoplasmic reticulum-to-nucleus signaling 1
      • Serine/threonine-protein kinase/endoribonuclease IRE1
      • ERN1
      • IRE1
      • Inositol-requiring protein 1
      • hIRE1p
      • Ire1-alpha
      • IRE1a
      Background InformationInositol-requiring protein 1 (IRE1p), also known as Ire1-alpha (IRE1a), Endoplasmic reticulum-to-nucleus signaling 1 (ERN1), and Serine/threonine-protein kinase/endoribonuclease IRE1, contains 1 KEN domain and 1 protein kinase domain.
      IRE1p has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1p is ubiquitously expressed with high levels observed in pancreatic tissue.
      References
      Product Information
      FormatAffinity Purified
      Control
      • A20 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Inositol-requiring protein 1 using this rabbit polyclonal antibody, Anti-IRE1p Antibody validated for use in western blotting, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected IRE1p in human pancreas and kidney tissues.

      Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected IRE1p in A20 cells.

      Immunofluorescence Analysis: 2 µg/mL from a representative lot detected IRE1p in A20 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human IRE1p.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the C-terminus of IRE1p.
      Species Reactivity
      • Mouse
      • Human
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • ERN1
      • IREp1
      Purification MethodAntigen Affinity Purified
      UniProt Number
      Molecular Weight~114 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A20 cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected IRE1p in A20 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN464 04053252912306