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MABF3201-25UL Anti-MART-1/Melan-A Antibody, clone A103

Anti-MART-1/Melan-A, clone A103, Cat. No. MABF3201, is a mouse monoclonal antibody that detects MART-1 and is tested for use in Immunohistochemistry and Western Blotting.

MABF3201-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3201-25UL
      DescriptionAnti-MART-1/Melan-A Antibody, clone A103
      Alternate Names
      • Melanoma antigen recognized by T-cells 1
      • Antigen LB39-AA
      • Antigen SK29-AA
      • Protein Melan-A
      Background InformationMelanoma antigen recognized by T-cells 1 (UniProt: Q16655; also known as MART-1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A) is encoded by the MLANA (also known as MART1) gene (Gene ID: 2315) in human. MART-1, a melanosome-specific protein, is a single-pass type III membrane protein with a transmembrane domain (aa 27-47) and a cytoplasmic domain (aa 48-118). Its expression is restricted to melanocytes, melanoma, and retina and is absent in other tissues and tumors. It is highly enriched in early melanosomes (Stage I and/or II melanosomes) indicating that its role in early melanogenesis. It is reported to stabilize both GPCR143/OA-1 and plays a role in the expression, stability, trafficking, and processing of pre-melanosome protein (PMEL) to ensure the formation of a stage 2 melanosome. It is mainly found in the endoplasmic reticulum (ER) and Golgi but is also detected in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation, which allows melanoma cells to be effectively recognized by specific cytotoxic T-cells. MART-1 may be a useful marker in the diagnosis of melanocytic lesions and in the differential diagnosis of malignant melanoma. (Ref.: Hoashi, T., et al. (2005). J. Biol. Chem. 280(14); 14006-14016; Chen, YT., et al. (1996). Proc. Natl. Acad. Sci. USA. 93(12); 5915-5919).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MART-1/Melan-A, clone A103, Cat. No. MABF3201, is a mouse monoclonal antibody that detects MART-1 and is tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesTested applications

      Western Blotting Analysis: A representative lot detected MART-1 in Western Blotting applicaiton. (Chen, Y.T., et al. (1996). Proc Natl Acad Sci USA. 93(12); 5915-9).

      Immunohistochemistry Applications: A representative lot detected MART-1/Melan-A in Immunohistochemistry applications (Chen, Y.T., et al. (1996). Proc Natl Acad Sci USA. 93(12);5915-9).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenFull-length, recombinant, human MART-1 produced in E.coli.
      EpitopeUnknown
      CloneA103
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone A103 is a mouse monoclonal antibody that detects MART-1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MLANA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~16 kDa observed; 13.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SK-MEL-28 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected MART-1 in SK-MEL-28 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3201-25UL 04065269800194