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ABC2036 Anti-MLKL

Anti-MLKL, Cat. No. ABC2036, is a rabbit polyclonal antibody that detects Mixed lineage kinase domain-like protein (MLKL) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-MLKL MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: MLKL UniProt Number: Q8NB16
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ABC2036
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC2036
      DescriptionAnti-MLKL
      Alternate Names
      • Mixed lineage kinase domain-like protein
      • hMLKL
      Background InformationMixed lineage kinase domain-like protein (UniProt: Q8NB16; also known as hMLKL) is encoded by the MLKL gene (Gene ID: 197259) in human. MLKL is a pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. MLKL contains a protein kinase-like domain; but it lacks several residues required for activity. Its ATP-binding site is located within amino acids 209-217. Two isoforms of MLKL have been described that are produced by alternative splicing. It contains two coiled coil regions (aa 55-84 and 139-180) and coiled coil region 2 is shown to be responsible for its homotrimerization. MLKL is phosphorylated on Thr357/Ser358 by RIP3 kinase that leads to its oligomerization. The oligomeric MLKL then translocates from cytosol to plasma membrane and intracellular membranes where it bind to phosphatidylinositol and cardiolipin and disrupts membrane integrity leading to necrotic cell death. MLKL is reported to play a critical role in TNF-induced necroptosis. (Ref.: Wang, H., et al (2014). Mol. Cell 54(1); 133-146).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MLKL, Cat. No. ABC2036, is a rabbit polyclonal antibody that detects Mixed lineage kinase domain-like protein (MLKL) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: A 1:500 dilution of from a representative lot detected MLKL in 10 µg of Jurkat cell lysate.

      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MLKL in human tonsil and human squamous cell carcinoma tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to the first 124 amino acids from the N-terminal region of human Mixed lineage kinase domain-like protein (MLKL).
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Mixed lineage kinase domain-like protein in human cells. It targets an epitope within the first 124 amino acids from the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MLKL
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~52 kDa observed; 54.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HT-29 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected MLKL in 10 µg of HT-29 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC2036 04054839415111