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MABN2726-100UG Anti-MSX1/2 Antibody, clone 4G1

Anti-MSX1/2, clone 4G1, Cat. No. MABN2726, is a mouse monoclonal antibody that detects Homeobox protein MSX-1/MSX-2 and is tested for use in Western Blotting, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.

MABN2726-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2726-100UG
      DescriptionAnti-MSX1/2 Antibody, clone 4G1
      Alternate Names
      • Homeobox protein MSX-2
      • Homeobox protein Hox-8
      • CHOX-8
      • GHox-8
      • Homeobox protein MSX-1
      • Homeobox protein Hox-7
      • CHOX-7
      • Msh homeobox 1-like protein
      Background InformationHomeobox protein MSX-2 (UniProt: P28362; also known as Homeobox protein Hox-8, CHOX-8, GHox-8) is encoded by the MSX2 (also known as HOX8) gene (Gene ID: 395245) in chicken. MSX2 acts as a transcriptional regulator in bone development and was first identified in craniofacial bone and human femoral osteoblasts. It is a homeodomain transcription factor and has a small DNA binding region (aa 134-193). MSX2 is predominantly localized in the nucleus, essential for its role in gene regulation. Its expressed in multiple tissues with notable expression in craniofacial mesenchyme, limb buds and neural crest cells. The expression pattern of MSX2 is tightly regulated and often overlaps with other MSX family members, indicating a complex regulatory network during development. It has a morphogenetic role and is involved in the shaping of structures during embryonic development. MSX2 regulates the differentiation and proliferation of cells, particularly in the craniofacial region, limbs, and neural crest cells. Genetic studies show that Msx2 and Dlx5 homeodomain (HD) proteins support skeletal development. Mutations or dysregulation of MSX2 can lead to several developmental anomalies and diseases: Craniosynostosis, Cleft palate and other craniofacial defects and limb malformations. While less studied in chickens, MSX2 has been implicated in cancer progression in humans, suggesting potential roles in tumorigenesis. Studies show that the MSX2-SOX2 signaling pathway plays a significant role in maintaining cancer stem cells, which are the origin of malignancy, leading to unfavorable outcomes in several carcinomas. (Ref.: Hassan, M.Q., et al. (2004). Mol Cell Biol. 24(20):9248-61; Roybon, L., et al. (2008). J Neurosci. 28(14):3644-56; Ko, H.W., et al. (2009). Methods Cell Biol. 93:347-69; Martinez-Morales, P.L., et al. (2011). J Cell Biol. 194(3):489-503; Thier, M.C., et al. (2019). Cell Stem Cell. 24(1):166-182.e13; Prasad, M.S., et al. (2020). Dev Biol. 458(1):64-74); Keyimu. R., et al. (2021). Exp Biol Med. 246(24): 2660 2670).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MSX1/2, clone 4G1, Cat. No. MABN2726, is a mouse monoclonal antibody that detects Homeobox protein MSX-1/MSX-2 and is tested for use in Western Blotting, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesTested Applications

      Immunohistochemistry Application: A representative lot detected MSX1/2 in Immunohistochemistry application (Ko, H.W., et al. (2009). Methods Cell Biol. 93:347-69; Martinez-Morales, P.L., et al. (2011). J Cell Biol. 194(3): 489-503; Prasad, M.S., et al. (2020). Dev Biol. 458(1): 64-74).

      Immunofluorescence Analysis: A representative lot detected MSX1/2 in Immunofluorescence application (Ko, H.W., et al. (2009). Methods Cell Biol. 93:347-69; Martinez-Morales, P.L., et al. (2011). J Cell Biol. 194(3): 489-503; Thier, M.C., et al. (2019). Cell Stem Cell. 24(1): 166-182.e13).

      Immunocytochemistry Analysis: A representative lot detected MSX1/2 in Immunocytochemistry application (Roybon, L., et al. (2008). J Neurosci. 28(14): 3644-56).

      Western Blotting Analysis: A representative lot detected MSX1/2 in Western Blotting application (Hassan, M.Q., et al. (2004). Mol Cell Biol. 24(20): 9248-61).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenFull Length, recombinant chicken MSX2.
      EpitopeUnknown
      Clone4G1
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 4G1 is a mouse monoclonal antibody that specifically detects Homeobox protein MSX2. This clone is also reactive to MSX1 (UniProt: P28361).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      • Chicken
      • Human
      Species Reactivity NoteHuman, Mouse, Rat, Chicken.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MSX2
      • MSX1
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~36 kDa observed; 28.24 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected Homeobox protein MSX1/MSX2 in Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2726-100UG 04065272163613