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MABN1540 Anti-P2RX7 Antibody, clone 14H7.1

Anti-P2RX7, clone 14H7.1, Cat. No. MABN1540, is a highly specific mouse monoclonal antibody that targets P2X purinoceptor 7 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-P2RX7 Antibody, clone 14H7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: P2RX7 UniProt Number: Q99572
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MABN1540
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1540
      DescriptionAnti-P2RX7 Antibody, clone 14H7.1
      Alternate Names
      • P2X purinoceptor 7
      • ATP receptor
      • P2Z receptor
      • Purinergic receptor
      Background InformationP2X purinoceptor 7 (UniProt: Q99572; also known as P2RX7, ATP receptor, P2Z receptor, Purinergic receptor) is encoded by the P2RX7 gene (Gene ID: 5027) in human. P2RX7 is a multi-pass membrane protein of the P2X receptor family that is widely expressed with highest levels reported in brain and immune tissues. Eight different isoforms of P2RX7 have been described that are generated via alternative splicing. Functional P2RX7 are shown to be organized as homomeric and as heteromeric trimers. They act as receptor for ATP and serve as a ligand-gated ion channel. They are also responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. They can function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. P2RX7 has two cytoplasmic domains (aa 1-25; 356-595); two transmembrane domains (aa 26-46; 335-355); and an extracellular domain (aa 47-334). P2RX7 can undergo phosphorylation that results in its inactivation. ADP-ribosylation at Arginine 125 is shown to be essential for its channel gating activity. Also, palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-P2RX7, clone 14H7.1, Cat. No. MABN1540, is a highly specific mouse monoclonal antibody that targets P2X purinoceptor 7 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected P2RX7 in human cerebellum and human tonsil tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 105 amino acids from the C-terminal half of human P2X purinoceptor 7.
      Clone14H7.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 14H7.1 detects P2X purinoceptor 7 in human cells. It targets an epitope within 105 amino acids from the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • P2RX7
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~69 kDa observed; 68.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human spleen tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected P2RX7 in 10 µg of human spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1540 04054839410505