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04-1460 Anti-PRMT5 Antibody, clone 23C7

Use Anti-PRMT5 Antibody, clone 23C7 (mouse monoclonal antibody) validated in WB to detect PRMT5 also known as 72 kDa ICCln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog.

Anti-PRMT5 Antibody, clone 23C7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins, RNA Metabolism & Binding Proteins Gene Symbol: PRMT5, HRMT1L5, IBP72, SKB1Hs, JBP1, SKB1 UniProt Number: O14744
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04-1460
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1460
      ReplacesMABE551
      DescriptionAnti-PRMT5 Antibody, clone 23C7
      Alternate Names
      • 72 kDa ICln-binding protein
      • HMT1 hnRNP methyltransferase-like 5
      • Jak-binding protein 1
      • SKB1 homolog
      • SKB1 homolog (S. pombe)
      • Shk1 kinase-binding protein 1 homolog
      • protein arginine methyltransferase 5
      • skb1 (S. pombe) homolog
      Background InformationArginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein.
      References
      Product Information
      FormatPurified
      Control
      • K562 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-PRMT5 Antibody, clone 23C7 (mouse monoclonal antibody) validated in WB to detect PRMT5 also known as 72 kDa ICCln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenRecombinant protein corresponding to human PRMT5.
      Clone23C7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes PRMT5.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PRMT5
      • HRMT1L5
      • IBP72
      • SKB1Hs
      • JBP1
      • SKB1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A/H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription.

      SIZE: 637 amino acids; 72684 Da

      SUBUNIT: Forms, at least, homodimers and homotetramers. Interacts with PRDM1 (By similarity). Component of the methylosome, a 20S complex containing at least pICLn, PRMT1/SKB1 and MEP50. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SUPT5H. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.

      SUBCELLULAR LOCATION: Cytoplasm.

      TISSUE SPECIFICITY: Ubiquitous.

      PTM: Disulfide bonds and non-covalent association mediate homooligomers formation.

      SIMILARITY: Belongs to the protein arginine N-methyltransferase family.
      Molecular Weight ~72 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected PRMT5 on 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      04-1460 04053252629136

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies