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ABT1389-50UL Anti-Phospho Lamin A/C (Ser22)

Anti-Phospho Lamin A/C (Ser22), Cat. No. ABT1389 is a rabbit polyclonal antibody that detects LAmin A/C phosphorylated on Serine 22 and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Anti-Phospho Lamin A/C (Ser22) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: LMNA, LMN1 UniProt Number: P02545
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ABT1389-50UL
50 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT1389-50UL
      DescriptionAnti-Phospho Lamin A/C (Ser22)
      Alternate Names
      • Prelamin A/C
      • Renal carcinoma antigen NY-REN-32
      Background InformationPrelamin-A/C (UniProt: P02545) is encoded by the LMNA (also known as LMN1) gene (Gene ID: 4000) in human. Prelamin-A/C is subsequently cleaved into Lamin A/C. Lamins are components of the nuclear lamina that provides a framework for the nuclear envelope and interact with chromatin. Prelamin-A/C
      Is cleaved to generate Lamin A/C. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting and subsequent cleavage by ZMPSTE24/FACE1 to remove the farnesyl group produces mature Lamin-A/C that is inserted into the nuclear lamina. Lamin A and C are present in equal amounts in the lamina of mammals and they play an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamins are shown to be essential for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Lamins also prevent fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone. Phosphorylation of Lamins is reported to occur continuously throughout all interphase periods and takes place mainly on the assembled lamina. Phosphorylation of the major polypeptides of the lamina induces laminar disassembly during mitosis. Phosphorylated Lamin-A/C localizes to nucleoplasm. Lamin A/C undergoes phosphorylation at multiple sites and one of the best characterized phosphorylation sites is on Serine 22 and it is phosphorylated during interphase. Phosphorylation of Serine 22 stabilizes Lamin A/C. Overexpression of Lamin-A is shown to result in greater phosphorylation of Serine 22 and 390 and Lamin A/C knockdowns display reduced phosphorylation at both sites, which helps in maintaining the integrity of the diminished lamina. Mutations in LMNA gene can cause Emery-Dreifuss muscular dystrophy 2 and 3, which are characterized by weakness and atrophy of muscle without involvement of the nervous system and cardiac conduction defects. Some mutations have also been linked to familial Lipodystrophy that leads to the loss of subcutaneous adipose tissue in the lower parts of the body and accumulation of adipose tissue in the face and neck. (Ref.: Buxboim, A., et al. (2014). Curr. Biol. 24(16): 1909-1917).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Phospho Lamin A/C (Ser22), Cat. No. ABT1389 is a rabbit polyclonal antibody that detects LAmin A/C phosphorylated on Serine 22 and has been tested for use in Western Blotting and Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A 1:1,000 dilution from a representative lot was used with A549 cells (specific for Lamin A/C phosphorylation) for peptide block analysis.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids from human Lamin A/C surrounding phosphorylated Serine 22.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects human Lamin A/C phosphorylated on Serine 22.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LMNA
      • LMN1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~75 kDa and 65 kDa observed; 74.14 and 65.14 kDa calculated for Lamin A and C, respectively. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Phospho Lamin A/C (Ser22) in A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT1389-50UL 04054839482250