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MABF824-25UL Anti-REG3A Antibody, clone 8B11.1

Anti-REG3A, clone 8B11.1, Cat. No. MABF824, is a mouse monoclonal antibody that detects Regenerating islet-derived protein 3-alpha (REG3A) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABF824-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF824-25UL
      DescriptionAnti-REG3A Antibody, clone 8B11.1
      Alternate Names
      • Regenerating islet-derived protein 3-alpha
      • REG-3-alpha
      • Hepatointestinal pancreatic protein
      • HIP/PAP
      • Human proislet peptide
      • Pancreatitis-associated protein 1
      • Regenerating islet-derived protein III-alpha
      • Reg III-alpha
      Background InformationRegenerating islet-derived protein 3-alpha (UniProt: Q06141; also known as REG-3-alpha, Hepatointestinal pancreatic protein, HIP/PAP, Human proislet peptide, Pancreatitis-associated protein 1, Regenerating islet-derived protein III-alpha, Reg III-alpha) is encoded by the REG3A (also known as PAP, PAP1) gene (Gene ID: 5068) in human. REG3-alpha is a member of the C-type lectin super family that exhibits bactericidal activity against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. It is a secretory protein that appears in pancreatic juice after induction of pancreatic inflammation and is shown to be highly induced during acute pancreatitis. In normal healthy pancreas, very low to undetectable expression is seen. It is also involved in hepatic and pancreatic regeneration. REG3-alpha is synthesized with a signal peptide (aa 1-26) and a propeptide (aa 27-37) that are cleaved off in the mature protein. Proteolytic processing by trypsin is shown to remove the inhibitory propeptide and this step is essential for peptidoglycan binding and antibacterial activity. REG3-alpha contain a C-type lectin domain (aa 47-172) and an EPN (Glu-Pro-Asn) motif (aa 114-116), The EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu114 playing a key role in peptidoglycan binding and bactericidal activity.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-REG3A, clone 8B11.1, Cat. No. MABF824, is a mouse monoclonal antibody that detects Regenerating islet-derived protein 3-alpha (REG3A) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected REG3A in human small intestine and human stomach tissue sections.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 128 amino acids from the internal region of human Regenerating islet-derived protein 3-alpha.
      Clone8B11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 8B11.1 is a mouse monoclonal antibody that detects human Regenerating islet-derived protein 3-alpha.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • REG3A
      • PAP
      • PAP1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~17 kDa observed; 19.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human small intestine tissue lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Regenerating islet-derived protein 3-alpha in Human small intestine tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF824-25UL 04054839500343

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