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ABC1453 Anti-Sodium-Iodine Symporter (NIS) C-term

Anti-Sodium-Iodine Symporter (NIS) C-term, Cat. No. ABC1453, is a rabbit polyclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

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ABC1453
50 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABC1453
      DescriptionAnti-Sodium-Iodine Symporter (NIS) C-term
      Alternate Names
      • Sodium/iodide cotransporter
      • Na(+)/I(-) cotransporter
      • NIS
      • Sodium-iodide symporter
      • Na(+)/I(-) symporter
      • Solute carrier family 5 member 5
      Background InformationSodium-Iodide Sympoter (Uniprot: Q63008; also known as NIS, Sodium/iodide cotransporter, Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter, Solute carrier family 5 member 5) is encoded by the Scl5a5 (also known as Nis) gene (Gene ID: 114613) in rat. NIS is a multi-pass membrane protein that is involved in the transport of iodide in thyroid gland. It is highly expressed in thyroid epithelial cells and lower levels of expression are also reported in mammary gland, salivary gland, stomach and colon. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. Nis gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Abnormalities in the expression of Nis gene lead to thyroid disease and can result in congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 2 M Tris, 0.2 M glycine buffer (pH 7.4) without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Sodium-Iodine Symporter (NIS) C-term, Cat. No. ABC1453, is a rabbit polyclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: A 1:1000-1:40K dilution from a representative lot detected Sodium-Iodine Symporter (NIS) C-term in FRTL-5, thyroid cells (Courtesy of Dr Nancy Carrasco & Dr. Andrea Reyna-Neyra at Yale University).

      Immunocytochemistry Analysis: A representative lot detected Sodium-Iodine Symporter (NIS) C-term in Immunocytochemistry applications (Levy, O., et. al. (1997). Proc Natl Acad Sci USA. 94(11):5568-73; De La Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).

      Immunohistochemistry Analysis: A representative lot detected Sodium-Iodine Symporter (NIS) C-term in rat thyroid, mammary gland from lactating rat (Tazebay, U.H., et. al. (2000). Nat Med. 6(8):871-8).

      Western Blotting Analysis: A representative lot detected Sodium-Iodine Symporter (NIS) C-term in Western Blotting applications (Levy, O., et. al. (1997). Proc Natl Acad Sci USA. 94(11):5568-73; Tazebay, U.H., et. al. (2000). Nat Med. 6(8):871-8; Levy, O., et. al. (1998). J Biol Chem. 273(35):22657-63; De La Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87).

      Immunoprecipitation Analysis: A representative lot detected Sodium-Iodine Symporter (NIS) C-term in Immunoprecipitation applications (De La Vieja, A., et. al. (2004). J Cell Sci. 117(Pt 5):677-87; Levy, O., et. al. (1998). J Biol Chem. 273(35):22657-63).
      Biological Information
      ImmunogenKLH-conjugated liner peptide corresponding to 16 amino acids from the C-termnus region of rat Sodium/iodide cotransporter.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects sodium/iodide co-transporter in rat thyroid gland. It targets an epitope within 16 amino acids from the C-terminus region.
      IsotypeIgG
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteMouse, Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Slc5a5
      • Nis
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~80 kDa observed; 65.20 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat thyroid tissue lysate.

      Western Blotting Analysis: 1.5 µg/mL of this antibody detected Sodium-Iodine Symporter (NIS) C-term in 10 µg of rat thyroid tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC1453 04054839233203

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