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MABE643 Anti-monomethyl Histone H3 (Lys79), clone RM147 Antibody

This Anti-monomethyl Histone H3 (Lys79), clone RM147 Antibody is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: H3F3A, H3.3A, H3F3, PP781, H3F3B, H3.3B UniProt Number: P84243
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MABE643
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, MplexRbPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE643
      DescriptionAnti-monomethyl Histone H3 (Lys79), clone RM147 Antibody
      Alternate Names
      • Histone H3.3
      • Histone H3
      Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. Lysine methylation occurs in three distinct states, having either one (me1), two (me2) or three (me3) methyl groups attached to the amine group of the lysine side chain. Methylation of Lysine 79 in Histone 3 acts as a marker of inactive chromatin regions. Methylation by Dot1 methylase on lysine-79 (Lys79) of Histone H3 is criticial for transcriptional silencing, and it is thought that silencing proteins such as Sir3 function by blocking Dot1 methylation.
      References
      Product Information
      FormatPurified
      PresentationRabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-monomethyl Histone H3 (Lys79), clone RM147 Antibody is validated for use in Western Blotting, Multiplexing for the detection of monomethyl Histone H3.
      Key Applications
      • Western Blotting
      • Multiplexing
      Application NotesMultiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
      Biological Information
      ImmunogenA linear peptide corresponding to human Histone H3 monomethylated at Lys79.
      CloneRM147
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis antibody reacts to Histone H3 monomethylated at Ly79 (K79me1). No cross reactivity with dimethylated Lys79 (K79me2) or trimethylated
      Lys79 (K79me3), or other methylations in histone H3.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • H3F3A
      • H3.3A
      • H3F3
      • PP781
      • H3F3B
      • H3.3B
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa acid extract and HeLa cell lysate.

      Western Blotting Analysis (WB): 0.5 μg/mL of this antibody detected monomethyl Histone H3 (Lys79) in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE643 04055977181456

      Documentation

      Anti-monomethyl Histone H3 (Lys79), clone RM147 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-monomethyl Histone H3 (Lys79), clone RM147 Antibody Certificates of Analysis

      TitleLot Number
      Anti-monomethyl Histone H3 (Lys79), clone RM147 -QVP1503206 QVP1503206
      Anti-monomethyl Histone H3 (Lys79), clone RM147 Monoclonal Antibody VP1712010

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.