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ABE265 Anti-phospho HuR (Ser221) Antibody

Anti-phospho HuR (Ser221) | ABE265 is an antibody against phospho HuR for use in Western Blotting, Peptide Inhibition Assay.

Anti-phospho HuR (Ser221) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: ELAVL1, HUR UniProt Number: Q15717
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ABE265
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE265
      DescriptionAnti-phospho HuR (Ser221) Antibody
      Alternate Names
      • ELAV-like protein 1
      • Hu-antigen R
      • HuR
      Background InformationHuR, also known as Human antigen R and ELAV-like protein 1 is encoded for by the gene ELAVL1. HuR, is a member of the embryonic lethal abnormal vision (ELAV) protein family that has been shown to regulate gene expression by stabilizing mRNA. HuR has been shown to bind to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of many target mRNAs. Stabilization of mRNA by HuR requires binding and export of nuclear HuR-mRNA complexes to the cytoplasm. In human cells this process is induced by angiotensin II (AngII) via Protein Kinase C delta (PKC-δ) triggered phosphorylation of HuR at Serines 221 and 318. Phosphorylation at Serine 221 which is located within the hinge domain of HuR was found to be critical for stimulus-induced export of HuR (Doller et al., 2010 Recent studies have shown that HuR is upregulated in certain cancers such as colon, breast and gastric cancers. HuR is also involved in embryonic stem cells (ESCs) differentiation. HuR preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, and thus promoting ESCs differentiation. HuR is also involved in the regulation of VEFG-A and NFkB expression in certain cell types. HuR activation and accumulation are also linked to many energy signaling molecules such as that of the PI3K/Akt pathway and AMPK (Doller et al., 2010. MOLECULAR AND CELLULAR BIOLOGY, Vol. 30, No. 6, p. 1397–1410).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho HuR (Ser221) | ABE265 is an antibody against phospho HuR for use in Western Blotting, Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A 1:200 dilution from a representative lot blocked phospho HuR (Ser221) in HCT116 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human phospho HuR (Ser221).
      EpitopePhosphorylated Ser221
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Bovine, Chicken, Porcine, Xenopus based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ELAVL1
      • HUR
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~37 kDa observed. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.

      Western Blotting Analysis: A 1:200 dilution of this antibody detected phospho HuR (Ser221) in 10 µg of HCT116 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE265 04055977123760

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies