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03-186 HDAC8 human recombinant

Human recombinant HDAC8 expressed in E. Coli.

HDAC8 human recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
03-186
100 Units  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsSpecies
      Enzyme AssaysHuman
      Description
      Catalogue Number03-186
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHDAC8 human recombinant
      OverviewHuman recombinant HDAC8 expressed in E. Coli
      Background InformationHDAC8 is phosphorylated in vivo by PKA, resulting in decreased activity and hyperacetylation of HDAC8 targets, such as histones H3 and H4. In addition, HDAC8 can also be inhibited by trichostatin A. HDAC8 has been implicated in acute myeloid leukemia.
      References
      Product Information
      FormatPurified
      PresentationIn liquid containing 10 mM Tris, pH 7.5, 100 mM NaCl, 3 mM MgCl2 and 10% glycerol. Frozen solution.

      Unit Definition: 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
      Quality LevelMQ300
      Applications
      ApplicationHuman recombinant HDAC8 expressed in E. Coli.
      Key Applications
      • Enzyme Assays
      Application NotesHDAC deacetylase assays
      Biological Information
      HostE. coli
      PurityPurity >50% by SDS-PAGE
      Specific Activity[Varies with lot. For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.]
      Molecular Weight~42 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC8 into separate tubes before storing at -80°C.
      Packaging Information
      Material Size100 Units
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      03-186 04053252550805

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      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes