Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Immobilon® Membranes, Sandwiches and Blotting Filter Paper
Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.More
Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications. Less
C3117Immobilon® PVDF membranes are the ideal transfer membranes for protein blotting applications.
Millipore offers four different Immobilon® PVDF (Polyvinylidene Difluoride) membranes, each optimized for different protein blotting applications. We also now offer blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper.
Immobilon® membranes provide a number of advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.
Immobilon®-P Membrane
0.45 µm pore size
Recommended for most western blotting, especially proteins >20 kDa
Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity
Immobilon®-E Membrane
The only PVDF membrane that wets out in water—no alcohol required
0.45 µm pore size ideal for most western blotting applications
Save time and reduce waste by eliminating the alcohol pre-wet step
Immobilon-PSQ Membrane
0.2 µm pore size and a large internal structure
Higher protein adsorption and sequencing yields than other
membranes
Recommended for blotting proteins <20 kDa
Prevents blow-through of low molecular weight proteins
Immobilon-FL Membrane
The first transfer membrane optimized for fluorescence applications
Extremely low background improves sensitivity of all fluorescence detection protocols
Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
Ideal for multiplexing and chemifluorescence applications
Immobilon® NOW Rolls & Dispenser
Convenience of cut sheets with the flexibility of rolls, offered for each Immobilon® PVDF membrane
Mini or midi size with a single cut
No wasted membrane
More compact package saves space in your lab
Blotting Sandwiches
When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon®-E or Immobilon®-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper.
Blotting Filter Paper
Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.
Performance
Rapid Immunodetection Protocol with Immobilon-P Membrane
Step
Standard Immunodetection
Rapid Immunodetection
1. Block the membrane
1 h
None
2. Incubate with primary antibody
1 h
1 h
3. Wash the membrane
3 x 10 min
3 x 5 min
4. Incubate with secondary antibody
1 h
30 min
5. Wash the membrane
3 x 10 min
3 x 5 min
6. Add substrate
5 min
5 min
Total time
4 h 5 min
2 h 5 min
Immobilon-PSQ Membrane Prevents Blow-through
Molecular weight standards (lanes 1, 3, 5, 7) and calf liver lysate (lanes 2, 4, 6, 8) were transferred to Immobilon-P or Immobilon-PSQ membranes. A sheet of Immobilon-PSQ was placed behind the primary membranes to capture proteins that passed through (lanes 5 and 6 behind Immobilon-P; lanes 7 and 8 behind Immobilon-PSQ).
Immobilon-FL Membrane is Optimized for Fluorescence
Actin-tubulin multiplex detection on Immobilon-FL membrane. Rabbit muscle actin (red) was detected using rabbit anti-actin 1oAB and QDot® 655 goat anti-rabbit 2oAB. Porcine brain tubulin (green) was detected using mouse anti-tubulin 1oAB and QDot 565 goat anti-mouse 2oAB. Sensitivities down to 7 ng were observed for both analytes. Data provided by Quantum Dot Corporation.
Immobilon® PVDF membranes are offered in three types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.
Features & Benefits
Won’t crack, curl or fracture when cut
Low background
Superior staining capabilities
Can be reprobed multiple times
Applications
Western Blotting, Dot Blotting, Protein Sequencing; Compatible with Radioactive, Chromogenic, Chemiluminescent, Fluorescent, and Chemifluorescent Detection
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