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420200 JC-1 - CAS 47729-63-5 - Calbiochem

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria.

JC-1 - CAS 47729-63-5 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide

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420200
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
47729-63-5C₂₅H₂₇Cl₄IN₄

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420200-5MG
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      		 Plastic ampoule 5 mg
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      Description
      OverviewA cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨm in cells, tissues, and isolated mitochondria. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. Ideal for detection of collapse of ΔΨm induced by uncouplers of mitochondrial respiration. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
      Catalogue Number420200
      Brand Family Calbiochem®
      Synonyms5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide
      References
      ReferencesMathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
      Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
      Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
      Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
      Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
      White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
      Ankacrona, M., et al. 1995. Neuron 15, 961.
      Reers, M., et al. 1995. Methods Enzymol. 260, 406.
      Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.
      Product Information
      CAS number47729-63-5
      ATP CompetitiveN
      FormRed-orange solid
      Hill FormulaC₂₅H₂₇Cl₄IN₄
      Chemical formulaC₂₅H₂₇Cl₄IN₄
      HS Code3204 90 00
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetUsed as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      420200-5MG 04055977210743

      Documentation

      JC-1 - CAS 47729-63-5 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      JC-1 - CAS 47729-63-5 - Calbiochem Certificates of Analysis

      TitleLot Number
      420200

      References

      Reference overview
      Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
      Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
      Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
      Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
      Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
      White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
      Ankacrona, M., et al. 1995. Neuron 15, 961.
      Reers, M., et al. 1995. Methods Enzymol. 260, 406.
      Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-July-2008 RFH
      Synonyms5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide
      DescriptionA cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨm in cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of ΔΨm induced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.
      FormRed-orange solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number47729-63-5
      Chemical formulaC₂₅H₂₇Cl₄IN₄
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.
      Toxicity Irritant
      ReferencesMathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
      Gravance, C.G., et al. 2000. Theriogenology 53, 1691.
      Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
      Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
      Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
      White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
      Ankacrona, M., et al. 1995. Neuron 15, 961.
      Reers, M., et al. 1995. Methods Enzymol. 260, 406.
      Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.