Millipore Sigma Vibrant Logo

71049 Proteinase K Solution, 600 mAU/ml

Efficient removal of proteins from nucleic acid solutions

Proteinase K Solution, 600 mAU/ml MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
71049
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
71049-3
Retrieving availability...
—
Fulfillment and delivery delayed Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Glass bottle 2 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      71049-4
      Retrieving availability...
      —
      Fulfillment and delivery delayed Fulfillment and delivery delayed
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Glass bottle 10 ml
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          Description
          OverviewProteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/ml for protein removal and enzyme inactivation, and up to 2 mg/ml for tissue treatment. The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the equivalent of 30 mAU (AU = Anson unit). The Novagen Proteinase K products are free of detectable DNase and RNase
          Catalogue Number71049
          Brand Family Novagen®
          References
          Product Information
          Unit of DefinitionOne AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.
          Quality LevelMQ400
          Applications
          Biological Information
          Physicochemical Information
          ContaminantsFree of detectable DNase and RNase.
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Harmful
          Storage -20°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          71049-3 07790788055455
          71049-4 07790788053000

          Documentation

          Proteinase K Solution, 600 mAU/ml Certificates of Analysis

          TitleLot Number
          71049

          Brochure

          Title
          High fidelity gene amplification
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

          Citations

          Title
        • Shannon M. Anderson, et al. (2007) New markers for murine memory B cells that define mutated and unmutated subsets. Journal of Experimental Medicine 204, 2103-2114.
        • Andrew A. Horwitz, et al. (2007) ATP-induced structural transitions in PAN, the proteasome-regulatory ATPase complex in Archaea. Journal of Biological Chemistry 282, 22921-22929.
          		•

          User Protocols

          Title
          TB271 Proteinase K

          Related Products & Applications

          Related Products

          Catalogue Number Description  
          70663 Proteinase K, Lyophilized Show Pricing & Availability

          Categories

          Life Science Research > Proteins and Enzymes > Other Enzymes
          Life Science Research > Genomic Analysis > DNA Preparation & Cloning > DNA Purification > DNA/RNA Clean-Up