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565758 β-Secretase Substrate IV, Fluorogenic - CAS 310427-94-2 - Calbiochem

β-Secretase Substrate IV, Fluorogenic, is a highly sensitive FRET substrate. Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement.

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Synonymes: FS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic

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565758
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      Description
      OverviewA highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement. kcat/Km values (min-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 are 0.044 and 0.040, respectively.
      Catalogue Number565758
      Brand Family Calbiochem®
      SynonymsFS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
      References
      ReferencesErmolieff, J., et al. 2000. Biochemistry 39, 12450.
      Product Information
      CAS number310427-94-2
      ATP CompetitiveN
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₉₁H₁₂₉N₂₅O₂₅S
      Chemical formulaC₉₁H₁₂₉N₂₅O₂₅S
      ReversibleN
      Quality LevelMQ100
      Applications
      Applicationβ-Secretase Substrate IV, Fluorogenic, is a highly sensitive FRET substrate. Hydrolysis of the substrate (Leu-Asp cleavage) by β-secretase results in fluorescence enhancement.
      Biological Information
      Primary Targetβ-secretase substrate
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Peptide SequenceH-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      565758-1MG 04055977267709

      Documentation

      β-Secretase Substrate IV, Fluorogenic - CAS 310427-94-2 - Calbiochem FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      β-Secretase Substrate IV, Fluorogenic - CAS 310427-94-2 - Calbiochem Certificats d'analyse

      TitreNuméro de lot
      565758

      Références bibliographiques

      Aperçu de la référence bibliographique
      Ermolieff, J., et al. 2000. Biochemistry 39, 12450.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-September-2008 RFH
      SynonymsFS-1, H-RE(EDANS)EVNLDAEFK(DABCYL)R-OH, Memapsin 2 Substrate I, Fluorogenic
      DescriptionA highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate (excitation max.: ~350 nm; emission max.: ~490), based on the GVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein. Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in fluorescence enhancement. Reported Kcat/km values (min-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu28p-mempasin 2 are 0.044 and 0.040, respectively (at pH 4.5, 37°C).
      FormRed lyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      CAS number310427-94-2
      Chemical formulaC₉₁H₁₂₉N₂₅O₂₅S
      Peptide SequenceH-Arg-Glu(EDANS)Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)Arg-OH
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Standard Handling
      ReferencesErmolieff, J., et al. 2000. Biochemistry 39, 12450.