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SCC153 9HTEo- Human Tracheal Epithelial Cell Line

9HTEo- human tracheal epithelial cell line is a useful model for the study of epithelial ion transport, secretion and biochemistry.

9HTEo- Human Tracheal Epithelial Cell Line : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Key Applications: Cell Culture, Cell Based Assays
SCC153
≥1X10⁶ cells/vial   
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      Replacement Information

      Tableau de caractéristiques principal

      Key Applications
      CULT, Cell Based Assays
      Description
      Catalogue NumberSCC153
      Description9HTEo- Human Tracheal Epithelial Cell Line
      Alternate Names
      • 9/HTEo-
      • 9/HTEo
      • 9HTE
      Background Information9HTEo- is a human tracheal epithelial cell line that was immortalized with the origin-of-replication defective SV40 plasmid (pSVori-). The cell line retains characteristic features of normal differentiated tracheal epithelial cells including a cobblestone morphology, cytokeratin expression and the ability to form tight junctions. 9HTEo- cells contain the wild-type cystic fibrosis transmembrane conductance regulator (WT-CFTR) and exhibits functional cAMP-dependent chloride ion transport and calcium ion-dependent chloride transport (1,2).

      9HTEo- is a useful model for the study of epithelial ion transport, secretion and biochemistry and frequently used as a wild-type control for human CF tracheobronchial epithelial cells such as CFBE41o- (Cat. No. SCC151) and CFTE29o- (Cat. No. SCC162).

      References:
      1. Gruenert DC, Basbaum CB, Welsh MJ, Li M, Finkbeiner WE, Nadel JA. (1988) Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus. Proc. Natl. Acad. Sci. USA 85: 5951-5955.
      2. Cozens AL, Yezzi MJ, Chin L, Simon EM, Finkbeiner WE, Wagner JA, Gruenert DC. (1992) Characterization of immortal cystic fibrosis tracheobronchial gland epithelial cells. Proc. Natl. Acad. Sci. USA 89(11): 5171-5175.
      References
      Product Information
      Applications
      Application9HTEo- human tracheal epithelial cell line is a useful model for the study of epithelial ion transport, secretion and biochemistry.
      Key Applications
      • Cell Culture
      • Cell Based Assays
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Epithelial Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X10⁶ viable cells.
      • Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, C, and HIV-1 & 2 viruses as assessed by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
      • Cells are negative for mycoplasma contamination.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      SCC153 04054839480447