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MABC1782-25UG Anti-MST1R/CD136 Antibody, clone 3G4

Anti-MST1R/CD136, clone 3G4, Cat. No. MABC1782, is a mouse monoclonal antibody that detects Macrophage-stimulating protein receptor and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blotting.

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MABC1782-25UG
25 μg  
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      Replacement Information
      Description
      Catalogue NumberMABC1782-25UG
      DescriptionAnti-MST1R/CD136 Antibody, clone 3G4
      Alternate Names
      • Macrophage-stimulating protein receptor
      • MSP receptor
      • CDw136
      • Protein-tyrosine kinase 8
      • p185-Ron
      • CD136
      Background InformationMacrophage-stimulating protein receptor (UniProt: Q04912; also known as EC:2.7.10.1, MSP receptor, CDw136, Protein-tyrosine kinase 8, p185-Ron, RON, CD136) is encoded by the MST1R (also known as PTK8, RON) gene (Gene ID: 4486) in human. CD136/RON, is a single-pass type I membrane glycoprotein of the MET family of receptors, is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-957), a transmembrane domain (aa 958-978), and a cytoplasmic domain (aa 979-1400). It is expressed as a 185-kDa glycosylated pro-form, which is proteolytically processed into a 35 kDa a-chain (aa 25-304) and 150 kDa b-chain (aa 310-1400) that are linked by disulfide bridges. The a-chain is shown to be fully extracellular while the b-chain is composed of an extracellular SEMA domain, three IPT domains, a transmembrane domain and an intracellular tyrosine kinase domain. CD136/RON expression has been reported in colon, skin, lung, and bone marrow. It serves as a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to macrophage stimulating 1 (MST1) ligand and regulates many physiological processes including cell survival, migration, and differentiation. Ligand binding on tyrosine 1238 and 1239 in the kinase domain is shown to induce its autophosphorylation, which leads to further phosphorylation on tyrosine 1353 and 1360 in the C-terminal multi-functional docking site. The activated form interacts with the PI3-kinase subunit PIK3R1, PLCG1, or the adapter GAB1 and the recruitment of these downstream effectors leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, and PLC g -PKC pathways. CD136 signaling is shown to activate the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. It also plays a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Mutations in MST1R gene are reported to cause nasopharyngeal carcinoma. Clone 3G4 binds to an epitope on the a-chain and detects a-chain and the pro form of CD136/RON. It is shown to bind strongly to deglycosylated CD136/RON in tunicamycin treated cancer cells. (Ref.: Koh, XY., et al. (2019). Oncogene. 38(48); 7342-7356).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MST1R/CD136, clone 3G4, Cat. No. MABC1782, is a mouse monoclonal antibody that detects Macrophage-stimulating protein receptor and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Flow Cytometry
      • Immunofluorescence
      • ELISA
      Application NotesTested Applications

      Western Blotting Analysis: A representative lot detected MST1R/CD136 in Western Blotting applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

      Immunocytochemistry Analysis: A representative lot detected MST1R/CD136 in Immunocytochemistry applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

      Immunofluorescence Analysis: A representative lot detected MST1R/CD136 in Immunofluorescence applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

      Flow Cytometry Analysis: A representative lot detected MST1R/CD136 in Flow Cytometry applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

      ELISA Analysis: A representative lot detected MST1R/CD136 in ELISA applications (Koh, X.Y., et. al. (2019). Oncogene. 38(48):7342-7356).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 238 amino acids from the extracellular domain of human RON/MST1R/CD136.
      EpitopeExtracellular domain
      Clone3G4
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 3G4 is a mouse monoclonal antibody that detects Macrophage-stimulating protein receptor (RON/MST1R/CD136). It targets an epitope within the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MST1R
      • PTK8
      • RON
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~35 and 152 kDa observed for a-chain and whole molecule, 152.24 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HT-29 cell lysate.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected MST1R/CD136 in HT-29 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABC1782-25UG 04065266298338