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OP01 Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259)

This Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections, Neutralization Studies for the detection of v-H-Ras (Ab-1).

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
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OP01
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Aperçu

Replacement Information

Tableau de caractéristiques principal

Species ReactivityHostAntibody Type
H, M, RRMonoclonal Antibody

Prix & Disponibilité

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OP01-100UG
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      		 Ampoule plast. 100 μg
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      Description
      OverviewRecognizes both phosphorylated and nonphosphorylated forms of v-H-Ras, and v-K-Ras in SW480 and Y1 cells and normal skin tissue. Also recognizes H, K, and N-Ras human translated products.
      Catalogue NumberOP01
      Brand Family Calbiochem®
      References
      ReferencesShimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlSW480 or Y1 cells or normal skin tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Neutralization Studies
      Paraffin Sections
      Application NotesFrozen Sections (5 µg/ml)
      Immunoblotting (2.5 µg/ml, colormetric or chemiluminescence)
      Immunofluorescence (2.5 µg/ml)
      Immunoprecipitation (use Cat. No. OP01A or 1 µg antibody per sample)
      Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required)
      Neutralization Studies (see application references)
      Application CommentsBroadly reactive with the phosphorylated and non-phosphorylated forms of v-H-Ras and v-K-Ras (see application references) and recognizes the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl v-H-ras (Ab-1) agarose conjugate (Cat. No. OP01A) is recommended to be used as an immunoconcentrator prior to immunoblot analysis of p21ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant p21 Ras
      Epitopewithin amino acids 62-76
      CloneY13-259
      HostRat
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      OP01-100UG 04055977209570

      Documentation

      Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Certificats d'analyse

      TitreNuméro de lot
      OP01

      Références bibliographiques

      Aperçu de la référence bibliographique
      Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-October-2007 RFH
      ApplicationFrozen Sections (5 µg/ml)
      Immunoblotting (2.5 µg/ml, colormetric or chemiluminescence)
      Immunofluorescence (2.5 µg/ml)
      Immunoprecipitation (use Cat. No. OP01A or 1 µg antibody per sample)
      Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required)
      Neutralization Studies (see application references)
      DescriptionPurified rat monoclonal antibody generated by immunizing Sprague Dawlay rats with the specified immunogen and fusing splenocytes with Y3Ag1.2.3 rat myeloma cells. Recognizes the ~21 kDa Ras protein (see comments).
      BackgroundOncogene sequences originally isolated from Harvey sarcoma virus v-H-ras and Kirsten sarcoma virus v-K-ras, both encode 21,000 dalton phosphoproteins (p21's) with guanine nucleotide binding activity. Human cellular homologues of v-H-ras and v-K-ras, designated c-H-ras and c-K-ras, respectively, and a related human oncogene, c-N-ras, each encode 21,000 dalton protein analogues to the gene products of their viral counterparts.
      HostRat
      Immunogenrecombinant p21 Ras
      Epitopewithin amino acids 62-76
      CloneY13-259
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlSW480 or Y1 cells or normal skin tissue
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsBroadly reactive with the phosphorylated and non-phosphorylated forms of v-H-Ras and v-K-Ras (see application references) and recognizes the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl v-H-ras (Ab-1) agarose conjugate (Cat. No. OP01A) is recommended to be used as an immunoconcentrator prior to immunoblot analysis of p21ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesShimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
      Taparowsky, E., et al. 1983. Cell 34, 581.
      Ellis, R.W., et al. 1981. Nature 292, 506.
      Shih, T.Y., et al. 1980. Nature 287, 686.
      Application referencesImmunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies