Millipore Sigma Vibrant Logo

HKI2MAG-99K MILLIPLEX MAP Human Kidney Injury Magnetic Bead Panel 2 - Toxicity Multiplex Assay

The analytes available for this multiplex kit are: α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, and Osteopontin (OPN).

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Toxicity Research Sub-Category: Toxicity
View Products on Sigmaaldrich.com
HKI2MAG-99K
Concevoir votre kit et Connaître son prix

Aperçu

Replacement Information

Tableau de caractéristiques principal

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
α-1-Microglobulin Albumin Clusterin Cystatin C EGF Lipocalin-2/NGAL Osteopontin (OPN) HMplexLuminex xMAP
Description
Catalogue NumberHKI2MAG-99K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Kidney Injury Magnetic Bead Panel 2 - Toxicity Multiplex Assay
Background InformationMaintenance of physiological balance in the body is attributed in part to normal kidney function. Altered kidney function due to injury, drug toxicity or kidney failure may be life threatening. Toxicity is the leading cause of drug failure, so research is expanding in search of more sensitive, rapid methods to determine organ-specific damage as quickly as possible. Traditionally, blood urea nitrogen (BUN) and serum creatinine tests have been used to determine drug-induced damage to the kidney. These tests only detect kidney damage after it begins and do not allow for the elucidation of where in the kidney the damage has occurred. Specificity and earlier detection of kidney injury is vital, and this area of research is expanding in search of more sensitive, rapid methods for determining the specific area of damage in kidney.

EMD Millipore provides valuable research assays to investigate multiple biomarkers of kidney injury in human urine samples using the Luminex xMAP® instrument platform.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 20%
  • Intra-assay: < 10%
Panel TypeMAGNETIC Toxicity
Quality LevelMQ200
Applications
ApplicationThe analytes available for this multiplex kit are: α-1-Microglobulin, Albumin, Clusterin, Cystatin C, EGF, Lipocalin-2/NGAL, and Osteopontin (OPN).
Key Applications
  • Multiplexing
Application Notes• Customers need to determine the optimal dilution factor for their samples. Our recommendations are based on urine samples from normal subjects.
• These assays require 25 µL diluted urine sample per well, sample dilution recommendations is 1:100 dilution in kit Assay Buffer
• An overnight (4°C) incubation is recommended.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • α-1-Microglobulin
  • Albumin
  • Clusterin
  • Cystatin C
  • EGF
  • Lipocalin-2/NGAL
  • Osteopontin (OPN)
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.
Accuracy
  • 95% - 109%
Physicochemical Information
Sensitivity
  • 0.015 - 5.359 ng/mL (MinDC = 2SD)
  • Please see kit protocol for individual assay sensitivities.
Standard Curve Range
  • α-1-Microglobulin: 0.98 – 1,000 ng/mL
  • Albumin: 3.91 – 4,000 ng/mL
  • Clusterin: 4.88 – 5,000 ng/mL
  • Cystatin C: 0.20 – 200 ng/mL
  • EGF: 0.01 – 10 ng/mL
  • Lipocalin-2/ NGAL: 0.02 – 20 ng/mL
  • Osteopontin (OPN): 0.59 – 600 ng/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
HKI2MAG-99K 04053252987403

Documentation

Protocols

Title
Protocol- HKI2MAG-99K

MILLIPLEX MAP Human Kidney Injury Magnetic Bead Panel 2 - Toxicity Multiplex Assay FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Brochure

Titre
The Power of Biomarker Analysis

Informations techniques

Titre
96 well Template 2 Up
96 well Template Single
HKI2MAG-99K xPonent Protocol Template Version Magpix
HKI2MAG-99K xPonent Standard Template Version Magpix
Quality Control Range Sheet- HKI2-109 and HKI2-209
Quality Control Range Sheet- HKI2-110 and HKI2-210
Quality Control Ranges: Milliplex Human Kidney Injury Magnetic Bead Panel 2

Posters

Titre
Toxicology in drug discovery and development: Biomarkers and model organisms