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71228 pSTBlue-1 AccepTor™ Vector Giga Kit - Novagen

Featuring super-efficient competent cells for increased transformation efficiency

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      Replacement Information
      Description
      Overview

      The pSTBlue-1 AccepTor™ Vector Giga Kits and pSTBlue-1 Perfectly Blunt® Giga Cloning Kits feature NovaBlue GigaSingles™ Competent Cells for increased transformation efficiency. AccepTor Vector Kits are designed for the cloning of PCR products generated using thermostable DNA polymerases that leave single 3′-dA nucleotide overhangs. The vector enables direct ligation by virtue of single 3′-dU overhangs that anneal with 3′-dA extensions on PCR products. The dU residues are converted to dT residues in vivo.

      The Perfectly Blunt Cloning Kits are designed for the cloning of DNA generated by PCR using any type of DNA polymerase. This allows for the use of high-fidelity proofreading enzymes for amplification, thereby decreasing the probability of generating mutations in the target sequence.
      Catalogue Number71228
      Brand Family Novagen®
      Features and benefits• No restriction enzymes or special primers
      • Direct ligation of PCR product with vector
      • Compatible with polymerases that leave single 3´-dA overhangs
      • Blue/white screening with pSTBlue-1 or pETBlue™-1 vectors
      • Simple protocol takes as little as 40 minutes from PCR product to plating transformants
      References
      Product Information
      Components
      DeclarationThis product is covered by U.S. Patent 5,856,144 owned by EMD Chemicals Inc. or its Affiliates.
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      71228 0

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      Produits associés classés par : Application Facete

      PCR and Cloning

      Produits associés classés par : Brand Facete

      AccepTor™

      Catégories

      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Kits