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Applications – Micronucleus Counting

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Reference Paper:
Multi-Parameter Dose Estimations in Radiation Biodosimetry Using the Automated Cytokinesis-Block Micronucleus Assay with Imaging Flow Cytometry. Cytometry A 2014; 10: 883-893.

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Merck:/Freestyle/BI-Bioscience/Cell-Analysis/amnis/Amins2-images/micronucleus-counting.jpgMicronuclei (MN) are formed as a result of DNA damage and appear as chromosomes or fragments of chromosomes that are not integrated into the daughter nuclei following cell division. The quantification of MN is widely used in toxicology to identify genotoxic compounds and as an indicator of radiation exposure in biological dosimetry.

The ImageStream®X overcomes many of the limitations that exist when performing the MN test by traditional flow cytometry and microscopy by rapidly capturing images from fluorescently stained cells in solution and automatically identifying and quantifying MN.

Quantifying Micronuclei in Irradiated Human Lymphocytes Using the ImageStream®X

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The cytokinesis-block micronucleus (CBMN) assay is a well-established technique in biological dosimetry for estimating radiation doses by correlating the frequency of MN in binucleated cells (BNCs) to a calibrated dose in peripheral blood lymphocytes. Advanced masking techniques allow for automated imaging, identification and enumeration of fluorescently stained BNCs and MN in irradiated lymphocytes. Using features such as aspect ratio, shape ratio and fluorescence spot counting, rapid analysis can be performed on large numbers of cell images captured by the ImageStream®X. Data was generously provided by M. A. Rodrigues.