Agarose-Based Affinity Purification

Protein A and Protein G are proteins of microbial origin that bind specifically but differently to mammalian immunoglobulins. When coupled to agarose, they provide an efficient tool for purification and immunoprecipitation (IP) of antibodies. Protein A agarose binds to the Fc region of IgG from a variety of species and can be used to purify classes, subclasses, and fragments of Igs and to isolate immune complexes. Protein G agarose is useful for binding to Igs that do not bind Protein A and can be used for antibody IP for purifying Igs and IgG fractions. Combining Protein A and Protein G agarose is a good strategy for exploiting the power of both Ig binding affinities in a single reagent.

Features & Benefits

• Agarose beads with either immobilized Protein A, Protein G, or a mixture of Protein A and G for optimal affinity
  
• Compatible with Amicon^® Pro purification system
  
• High binding capacity and low ligand leaching
  
• Two convenient formats, one for purification in column or batch mode and the other is a ready to-use suspension containing BSA for IP applications

Applications

Immunoprecipitation, Affinity Purification, Antibody Purification

Product Performance